Anti-Sca-1 antibody-functionalized vascular grafts improve vascular regeneration via selective capture of endogenous vascular stem/progenitor cells

作     者：He Wang Mengmeng Xing Weiliang Deng Meng Qian Fei Wang Kai Wang Adam C.Midgley Qiang Zhao 

作者机构：State Key Laboratory of Medicinal Chemical BiologyHaihe Laboratory of Sustainable Chemical TransformationsKey Laboratory of Bioactive Materials(Ministry of Education)Frontiers Science Center for Cell ResponsesCollege of Life SciencesNankai UniversityTianjinChina 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第10期

页      面：433-450页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Nos.81925021 82050410449 81921004 and 81871500)and Science&Technology Project of Tianjin of China(No.18JCJQJC46900) 

主　　题：Small-diameter vascular grafts Surface modification Stem cell antigen-1(Sca-1)antibody Vascular stem/progenitor cells(SPCs) Tissue regeneration 

摘      要：Small-diameter vascular grafts fabricated from synthetic biodegradable polymers exhibit beneficial mechanical properties but often face poor regenerative *** tissue engineering approaches have been employed to improve tissue regeneration in vascular grafts,but there remains a requirement for a new generation of synthetic grafts that can orchestrate the host response to achieve robust vascular *** stem/progenitor cells(SPCs)are mostly found in quiescent niches but can be activated in response to injury and participate in endothelium and smooth muscle regeneration during neo-artery ***,we developed a functional vascular graft by surface immobilization of stem cell antigen-1(Sca-1)antibody on an electrospun poly(ε-caprolactone)graft(PCL-Sca-1 Ab).PCL-Sca-1 Ab promoted capture and retainment of Sca-1+SPCs in *** rat abdominal aorta replacement models,PCL-Sca-1 Ab stimulated in vivo recruitment of Sca-1+SPCs,and drove SPCs differentiation towards vascular cell *** origin of infiltrated Sca-1+SPCs was further investigated using a bone marrow transplantation mouse model,which revealed that Sca-1+SPCs originating from the resident tissues and bone marrow contributed to rapid vascular regeneration of vascular *** data indicated that PCL-Sca-1 Ab vascular grafts may serve as a useful strategy to develop next generation cell-free vascular grafts.