Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920

作     者：杨婵媛 朱晓丽 范代娣 米钰 骆艳娥 惠俊峰 苏然 

作者机构：Shaanxi Key Laboratory of Degradable Biomedical MaterialsSchool of Chemical EngineeringNorthwest University Shaanxi R&D Center of Biomaterials and Fermentation EngineeringSchool of Chemical EngineeringNorthwest University Shaanxi R&D Center of Biomaterials and Fermentation EngineeringSchool of Urban and Environmental ScienceNorthwest University 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2012年第20卷第5期

页      面：930-936页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0817[工学-化学工程与技术] 08[工学] 0703[理学-化学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：Supported by the National High Technology Research and Development Program of China (2007AA03Z456) the National Natural Science Foundation of China (20776119, 21076169 and 31000019) the Xi’an Research and Development Program(NC08005, YF07078) the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109) Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03) the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014) NWU Graduate Innovation and Creativity Funds (08YSY17) Shaanxi Key Subject Program, China 

主　　题：Bifidobacterium freeze-drying viability protective agent response surface methodology 

摘      要：Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement (10 6 cfu·g-1 ).