Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats

作     者：Zheng-Yu Yao Xiao-Huan Li Li Zuo Qian Xiong Wen-Ting He Dong-Xu Li Zhi-Fang Dong Zheng-Yu Yao;Xiao-Huan Li;Li Zuo;Qian Xiong;Wen-Ting He;Dong-Xu Li;Zhi-Fang Dong

作者机构：Pediatric Research InstituteMinistry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2022年第43卷第3期

页      面：380-390页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(82071395,91749116) Natural Science Foundation of Chongqing(cstc2021ycjh-bgzxm0186,cstc2020jcyjzdxm X0004) Science and Technology Research Program of Chongqing Municipal Education Commission(KJZD-K201900403) Innovation Research Group at Institutions of Higher Education in Chongqing(CXQTP19034) 

主　　题：Maternal sleep deprivation Gut microbiota Neuroinflammation Gut-brain axis Cognitive function Firmicutes 

摘      要：Maternal sleep deprivation(MSD)is a global public health problem that affects the physical and mental development of pregnant women and their *** latest research suggests that sleep deprivation(SD)disrupts the gut microbiota,leading to neuroinflammation and psychological ***,it is unclear whether MSD affects the establishment of gut microbiota and neuroinflammation in the *** the present study,MSD was performed on pregnant SpragueDawley rats in the third trimester of pregnancy(gestational days 15-21),after which intestinal contents and brain tissues were collected from offspring at different postnatal days(P1,P7,P14,and P56).Based on microbial profiling,microbial diversity and richness increased in pregnant rats subjected to MSD,as reflected by the significant increase in the phylum *** addition,microbial dysbiosis marked by abundant Firmicutes bacteria was observed in the MSD ***,quantitative real-time polymerase chain reaction(q RT-PCR)and enzyme-linked immunosorbent assay(ELISA)showed that the expression levels of proinflammatory cytokines interleukin 1β(IL-1β)and tumor necrosis factorα(TNF-α)were significantly higher in the MSD offspring at adulthood(P56)than in the control *** Spearman correlation analysis,IL-1βand TNF-αwere also shown to be positively correlated with Ruminococcus_1 and Ruminococcaceae_UCG-005 at P56,which may determine the microbiota-host interactions in MSDrelated ***,these results indicate that MSD changes maternal gut microbiota and affects the establishment of neonatal gut microbiota,leading to neuroinflammation in MSD ***,understanding the role of gut microbiota during physiological development may provide potential interventions for cognitive dysfunction in MSD-impacted offspring.