Polydopamine-modified chitin conduits with sustained release of bioactive peptides enhance peripheral nerve regeneration in rats

作     者：Ci Li Song-Yang Liu Li-Ping Zhou Tian-Tian Min Meng Zhang Wei Pi Yong-Qiang Wen Pei-Xun Zhang Ci Li;Song-Yang Liu;Li-Ping Zhou;Tian-Tian Min;Meng Zhang;Wei Pi;Yong-Qiang Wen;Pei-Xun Zhang

作者机构：Department of Orthopedics and TraumaPeking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural RegenerationMinistry of EducationBeijingChina National Center for Trauma MedicineBeijingChina Beijing Key Laboratory for Bioengineering and Sensing TechnologySchool of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第11期

页      面：2544-2550页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071006[理学-神经生物学] 

基　　金：the National Natural Science Foundation of China,Nos.31771322,31571235 the Natural Science Foundation of Beijing,No.7212121 Beijing Science Technology New Star Cross Subject of China,No.2018019 Shenzhen Science and Technology Plan Project of China,No.JCYJ 20190806162205278 the Key Laboratory of Trauma and Neural Regeneration(Peking University),Ministry of Education a grant from National Center for Trauma Medicine,No.BMU2020XY005-01(all to PXZ)。 

主　　题：angiogenesis bioactive peptides nerve repair neurotrophic factor peripheral nerve injury peripheral nerve regeneration polydopamine surface modification synergistic effects tissue engineering 

摘      要：The introduction of neurotrophic factors into injured peripheral nerve sites is beneficial to peripheral nerve regeneration.However,neurotrophic facto rs are rapidly degraded in vivo and obstruct axonal regeneration when used at a supraphysiological dose,which limits their clinical benefits.Bioactive mimetic peptides have been developed to be used in place of neurotrophic factors because they have a similar mode of action to the original growth fa ctors and can activate the equivalent receptors but have simplified sequences and structures.In this study,we created polydopamine-modified chitin conduits loaded with brain-derived neurotrophic factor mimetic peptides and vascular endothelial growth fa ctor mimetic peptides(Chi/PDA-Ps).We found that the Chi/PDA-Ps conduits were less cytotoxic in vitro than chitin conduits alone and provided sustained release of functional peptides.In this study,we evaluated the biocompatibility of the Chi/P DA-Ps conduits.Brain-derived neurotrophic factor mimetic peptide and vascular endothelial growth fa ctor mimetic peptide synergistically promoted prolife ration of Schwann cells and secretion of neurotrophic factors by Schwann cells and attachment and migration of endothelial cells in vitro.The Chi/P DA-Ps conduits were used to bridge a 2 mm gap between the nerve stumps in rat models of sciatic nerve injury.We found that the application of Chi/PDA-Ps conduits could improve the motor function of rats and reduce gastrocnemius atrophy.The electrophysiological results and the microstructure of regenerative nerves showed that the nerve conduction function and re myelination was further resto red.These findings suggest that the Chi/PDA-Ps conduits have great potential in peripheral nerve injury repair.