Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds
作者机构：Robert W.Holley Center for Agriculture and HealthUSDA-ARSCornell UniversityIthacaNY 14853USA Plant Breeding and Genetics SectionSchool of Integrative Plant ScienceCornell UniversityIthacaNY 14853USA State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Department of Biochemistry and Center for Plant Science InnovationUniversity of Nebraska-LincolnLincolnNE 68588USA
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2021年第2卷第3期
页 面：191-214页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：the USDA National Institute of Food and Agriculture(NIFA) to LL and the USDA-ARS fund EBC was supported by a grant from USDA NIFA supported by the Agriculture and Food Research Initiative competitive
主 题：Carotenoid PSY OR^(His) HGGT BCH2 Seed Metabolic engineering
摘 要：Staple grains with low levels of provitamin A carotenoids contribute to the global prevalence of vitamin A deficiency and therefore are the main targets for provitamin A ***,carotenoid stability during both seed maturation and postharvest storage is a serious concern for the full benefits of carotenoid biofortified *** this study,we utilized Arabidopsis as a model to establish car-otenoid biofortification strategies in *** discovered that manipulation of carotenoid biosynthetic activity by seed-specific expression of Phytoene synthase(PSY)increases both provitamin A and total carotenoid levels but the increased carotenoids are prone to degradation during seed maturation and storage,consistent with previous studies of provitamin A biofortified *** contrast,stacking with Orange(OR^(His)),a gene that initiates chromopl ast biogenesis,dramatically enhances provitamin A and total carotenoid content and *** to 65-and 10-fold increases of β-carotene and total car-otenoids,res pectively,with provitamin A carotenoids composing over 63%were observed in the seeds containing OR^(His) and ***-expression of Homogen tisate geranylgeranyl transferase(HGGT)with OR^(His) and PSY further increases carotenoid accumulation and stability during seed maturation and ***,knocking-out of B-carotene hydroxylase 2(BCH2)by CRISPR/Cas9 not only potentially facilitates β-carotene accumulation but also minimizes the negative effect of carotenoid over production on seed germi *** findings provide new insights into various processes on carotenoid accu-mulation and stability in seeds and establish a multiplexed strategy to simultaneously target carotenoid biosynthesis,turnover,and stable storage for carotenoid biofortification in crop seeds.
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