ISSR Analysis of Chlorophytum Treated by Three Kinds of Chemical Mutagen

作     者：WU Lijun LI Muzi YANG Xue YANG Tao WANG Jingang 

作者机构：College of Horticulture Northeast Agricultural University Harbin 150030 China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2011年第18卷第4期

页      面：21-25页

学科分类：0710[理学-生物学] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by Heilongjiang Provincial Youth Science and Technology Fund (QC07C51) Innovation Project of Northeast Agricultural University (CX2004-2) 

主　　题：Chlorophytum chemical mutagenesis ISSR 

摘      要：Chlorophytum leaves were treated with three kinds of chemical mutagen, EMS, DES and NaN3 with different concentration to obtain the variation materials with excellent properties. The results showed that the genetic similarity coefficient of variation plants with EMS treatment was between 0.648 and 0.868, with NaN3 treatment was between 0.598 and 0.859, and with DES treatment was between 0.668 and 0.904, of which the mutagenic effects with 0.8% EMS, 250 mg. L-1 NaN3 and 0.3% DES on chloro- phytum were the best. ISSR molecular marker technique was used to analyze their genetic diversities. Total 392 polymorphic bands were obtained through 18 ISSR primers. Polymorphic ratio was 72.4%, which showed that DNA mutation took place in various degrees