Apelin aggravates the migration and invasion of non-small cell lung cancer cells via YAP1

作     者：Wenchao Ma Di Zhu Tong Yu Junwen Xue Xiang Sun Guofang Zhang Yumeng Hou Jinrui Li Yingzhun Chen Hongli Shan Haihai Liang Yumeng Hou;Jinrui Li;Yingzhun Chen;Hongli Shan;Haihai Liang

作者机构：State-Province Key Laboratories of Biomedicine-Pharmaceutics of ChinaKey Laboratory of Cardiovascular ResearchMinistry of EducationDepartment of PharmacologyCollege of PharmacyHarbin Medical UniversityHarbin 150081China Northern Translational Medicine Research and Cooperation CenterHeilongjiang Academy of Medical SciencesHarbin Medical UniversityHarbin 150081China Department of Pathologythe 2nd Affiliated HospitalHarbin Medical UniversityHarbin 150081China Research Unit of Noninfectious Chronic Diseases in Frigid Zone(2019RU070)Chinese Academy of Medical SciencesHarbin 150081China 

出 版 物：《Frigid Zone Medicine》 (寒地医学（英文）)

年 卷 期：2022年第2卷第1期

页      面：53-64页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(81872863) the Major Scientific Fund Project of Heilongjiang Province(ZD2019H001) 

主　　题：non-small cell lung cancer apelin yes associated protein 1 epithelial-mesenchymal transition 

摘      要：Background:Apelin,an endogenous ligand of G-protein coupled receptor(GPCR),is a secreted peptide involved in the development of various ***,the relationship between apelin and non-small cell lung cancer(NSCLC)is not quite *** study was designed to investigate the effect and mechanism of apelin on cell proliferation,migration and invasion of NSCLC ***:Twelve NSCLC specimens were collected for hematoxylin-eosin(HE)staining and immunohistochemistry *** proliferation was examined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and cell migration and invasion were assessed using wound-healing and transwell *** subcellular location of yes associated protein 1(YAP1)in A549 cells was determined by *** mRNA and protein levels in NSCLC tissues and cell lines were measured by qRT-PCR and western blot,***:Apelin was upregulated in tumor tissues compared with the adjacent *** promoted proliferation,migration,and invasion of A549 and H460 cells,which was reversed by competitive apelin receptor(APJ)antagonist ***,apelin upregulated YAP1 expression,whereas silence of YAP1 by small interfering RNA(siRNA)attenuated apelin-induced cell proliferation,migration and invasion and suppressed epithelial-mesenchymal transition ***:Apelin promotes NSCLC cells proliferation,migration,and invasion by modulating YAP1 and might be a potential therapeutic target for NSCLC treatment.