Indigenous microbiota protects development of medication-related osteonecrosis induced by periapical disease in mice

作     者：Wen Du Mengyu Yang Terresa Kim Sol Kim Drake W.Williams Maryam Esmaeili Christine Hong Ki-Hyuk Shin Mo K.Kang No-Hee Park Reuben H.Kim Wen Du;Mengyu Yang;Terresa Kim;Sol Kim;Drake W.Williams;Maryam Esmaeili;Christine Hong;Ki-Hyuk Shin;Mo K.Kang;No-Hee Park;Reuben H.Kim

作者机构：State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of ProsthodonticsWest China Hospital of StomatologySichuan UniversityChengduChina The Shapiro Family Laboratory of Viral Oncology and Aging ResearchLos AngelesUSA Section of Restorative DentistryUCLA School of DentistryLos AngelesUSA Department of Orofacial SciencesUCSF School of DentistrySan FranciscoUSA UCLA Jonsson Comprehensive Cancer CenterLos AngelesUSA David Geffen School of Medicine at UCLALos AngelesUSA 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2022年第14卷第2期

页      面：156-163页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：supported National Institutes of Health/National Institute of Dental and Craniofacial Research(grant DE023348 to R.H.K.,grant DE025172 to D.W.W.) China Postdoctoral Science Foundation(grant 2019M663526 to W.D.) 

主　　题：protective finding antibiotics 

摘      要：Bacterial infection is a common finding in patients,who develop medication-related osteonecrosis of the jaw(MRONJ)by the longterm and/or high-dose use of anti-resorptive agents such as bisphosphonate(BPs).However,pathological role of bacteria in MRONJ development at the early stage remains ***,we demonstrated that commensal microbiota protects against MRONJ development in the pulp-exposed periapical periodontitis mouse model.C57/BL6 female mice were treated with intragastric broadspectrum antibiotics for 1 *** acid(ZOL)through intravenous injection and antibiotics in drinking water were administered for throughout the *** was exposed on the left maxillary first molar,then the mice were left for 5 weeks after which bilateral maxillary first molar was extracted and mice were left for additional 3 weeks to *** mice were harvested,and cecum,maxilla,and femurs were *** development was assessed usingμCT and histologic *** antibiotic was treated in mice,these mice had no weight changes,but developed significantly enlarged ceca compared to the control group(CTL mice).Periapical bone resorption prior to the tooth extraction was similarly prevented when treated with antibiotics,which was confirmed by decreased osteoclasts and *** treatment with pulp exposure significantly increased bone necrosis as determined by empty lacunae and necrotic bone ***,antibiotics treatment could further exacerbate bone necrosis,with increased osteoclast *** findings suggest that the commensal microbiome may play protective role,rather than pathological role,in the early stages of MRONJ development.