Colorimetric and electrochemical detection of ligase through ligation reaction-induced streptavidin assembly

作     者：Yaliang Huang Ting Sun Wendi Li Lin Liu Gang Liu Xinyao Yi Jianxiu Wang Yaliang Huang;Ting Sun;Wendi Li;Lin Liu;Gang Liu;Xinyao Yi;Jianxiu Wang

作者机构：Hunan Provincial Key Laboratory of Micro&Nano Materials Interface ScienceCollege of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China Henan Province of Key Laboratory of New Optoelectronic Functional MaterialsAnyang Normal UniversityAnyang 455000China Sanquan College of Xinxiang Medical UniversityXinxiang 453003China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第6期

页      面：3151-3155页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：the National Natural Science Foundation of China(Nos.22076221,21876208) the Program for Innovative Research Team of Science and Technology in the University of Henan Province(No.21IRTSTHN005) the Hunan Provincial Science and Technology Plan Project,China(No.2019TP1001) 

主　　题：Ligase Electrochemical analysis Streptavidin Gold nanoparticles Colorimetric assay Electrochemical impedance spectroscopy 

摘      要：We propose a concept for ligase detection by conversion of aggregation-based homogeneous analysis into surface-tethered electrochemical assay through streptavidin(SA)-biotin *** A(Srt A)served as the model analyte and two biotinylated peptides(bio-LPETGG and GGGK-bio)were used as the *** A-catalyzed ligation of the peptide substrates led to the generation of *** ligation product(bio-LPETGGGK-bio)induced the aggregation and color change of SA-modified gold nanoparticles(Au NPs)through the SA-biotin interactions,which could be assayed by the colorimetric ***,we found that the bio-LPETGGGK-bio could trigger the assembly of tetrameric SA proteins with the formation of the(SA-bio-LPETGGGK-bio)nassemblies through the same *** above results were further confirmed by atomic force microscopy and fluorescent *** insulated assemblies were in-situ fabricated at the SA-modified gold electrode,thus hindering the electron transfer of[Fe(CN)_(6)]^(3-/4-) and leading to an increase in the electron-transfer *** capability of the method for the detection of Srt A both in vitro and Staphylococcus aureus(***)has been *** A with a concentration down to 1 pmol/L has been determined by the electrochemical analysis,which is lower than that achieved by the colorimetric assay(50 pmol/L).By integrating the advantages of homogeneous reaction and heterogeneous detection,the strategy serves as an ideal means for the fabrication of various sensing platforms by adopting biotin-labeled and sequence-specific peptide or nucleic acid substrates.