The comparative analysis and identification of secondary metabolites between Tibet wild and cultivated pomegranates(Punica granatum L.)in China

作     者：GUO Lin-hui GE Da-peng REN Yuan DONG Jian-mei ZHAO Xue-qing LIU Xue-qing YUAN Zhao-he GUO Lin-hui;GE Da-peng;REN Yuan;DONG Jian-mei;ZHAO Xue-qing;LIU Xue-qing;YUAN Zhao-he

作者机构：Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037P.R.China College of ForestryNanjing Forestry UniversityNanjing 210037P.R.China Yantai Academy of Agricultural SciencesYantai 265500P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第3期

页      面：736-750页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the Natural Science Foundation of Tibet Autonomous Region,China(XZ201019ZRG-153) the Initiative Project for Talents of Nanjing Forestry University,China(GXL2014070 and GXL2018032) the National Natural Science Foundation of China(31901341) the Natural Science Foundation of Jiangsu Province,China(BK20180768) the Priority Academic Program Development of Jiangsu High Education Institutions,China(PAPD)。 

主　　题：wild pomegranate secondary metabolites flavonoid tannin LC–ESI-MS/MS 

摘      要：Secondary metabolites are closely related to the nutritional quality and health functions of plants.We investigated the secondary metabolites of both wild(n=23)and cultivated(n=27)pomegranate plants(Punica granatum L.)growing in China.The total flavonoid(TF)and tannin(TT)contents from the peel and juice were determined and the secondary metabolites in the peel(ZLP)and juice(ZLZ)of‘Zela 4’were identified using liquid chromatography–electrospray ionization tandem mass spectrometry(LC–ESI-MS/MS).Analysis of variance(P0.05)showed that there were significant differences in the TF content of peel(TF(P))and juice(TF(J)),and the TT content of peel(TT(P))and juice(TT(J))among different pomegranate accessions.Pearson correlation analysis showed that latitude and altitude might be the main environmental factors affecting TF and TT contents in pomegranates.In this study,279 secondary metabolites were identified in the ZLP and ZLZ.In addition,we report for the first time 227 secondary metabolites in pomegranates.Using orthogonal partial least squares discriminant analysis,90 differential metabolites were identified in ZLP and ZLZ.In addition,we screened eight specific germplasms(high-TF(P),‘Junyong 3’;low-TF(P),‘Yanzhihong’;high-TF(J),‘Zela 4’;low-TF(J),‘Yudazi’,high-TT(P),‘Junyong 4’;low-TT(P),‘Anba 1’;high-TT(J),‘Yeba 1’;and low-TT(J),‘Baihuayushizi’).The results of our study provide a reference for the development and utilization of wild pomegranate resources and pomegranate breeding in China.