Kinetic characterization of a slow chemical exchange between two sites in N,N-dimethylacetylamide by CEST NMR spectroscopy

作     者：Lixia Wang Jikun Li Junfeng Xiang Jie Cui Yalin Tang Lixia Wang;Jikun Li;Junfeng Xiang;Jie Cui;Yalin Tang

作者机构：Beijing National Laboratory for Molecular Sciences(BNLMS)Center for Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第9期

页      面：4335-4338页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported under the National Natural Science Foundation of China(Nos.22077123 and 21977099) 

主　　题：Chemical exchange saturation transfer(CEST) Chemical exchange N,N-Dimethylacetylamid Bloch-Mc Connell equation Dynamic kinetics 

摘      要：Nuclear magnetic resonance(NMR)spectroscopy has provided many powerful tools for the study of dynamic *** the reported methods,chemical exchange saturation transfer(CEST)is more suitable for systems with slow exchange rates,and there will be promising in the detection and dynamic mechanism of metastable *** has been widely used in magnetic resonance imaging(MRI),however whether it is applicable in the field of chemical kinetics needs more *** we studied,as a proof of concept,the kinetics of the slow chemical exchange between the two N-methyl protons of N,N-dimethylacetylamide(DMA),exploiting QUantifying Exchange using Z-spectrum(QUEZS)and QUantifying Exchange using Saturation Time(QUEST)*** turned out that both of QUEZS and QUEST could give the corresponding exchange rates,showcasing the capability of this method to provide accurate kinetic data under a range of *** results clearly demonstrated the reliability of CEST-based techniques as a tool for dynamic kinetics by NMR.