Development of genomic resources for Wenchengia alternifolia(Lamiaceae)based on genome skimming data

作     者：Qi-Yue Zhou Hui-Xia Cai Zi-Han Liu Lang-Xing Yuan Lei Yang Tuo Yang Bo Li Pan Li Qi-Yue Zhou;Hui-Xia Cai;Zi-Han Liu;Lang-Xing Yuan;Lei Yang;Tuo Yang;Bo Li;Pan Li

作者机构：Laboratory of Systematic&Evolutionary Botany and BiodiversityCollege of Life SciencesZhejiang UniversityHangzhou310058China Shanghai Key Laboratory of Plant Functional Genomics and ResourcesShanghai Chenshan Botanical GardenShanghai201602China Haikou Duotan Wetlands InstituteHaikou570100China Orchid Conservation&Research Center of ShenzhenShenzhen518114China Research Centre of Ecological SciencesCollege of AgronomyJiangxi Agricultural UniversityNanchang330045China 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2022年第44卷第6期

页      面：542-551页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 0713[理学-生态学] 

基　　金：the National Natural Science Foundation of China(grant number 31970225 and 31900181) the Zhejiang Provincial Natural Science Foundation(grant number LY19C030007) the Open Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources(PFGR202104) 

主　　题：Wenchengia Plastid hotspot Simple sequence repeat(SSR) Single nucleotide polymorphism(SNP) 

摘      要：Wenchengia alternifolia(Lamiaceae),the sole species of the genus Wenchengia is extremely rare and is currently listed as Critically Endangered(CR) on the IUCN Red List. The species had long been considered endemic to Hainan Island,China and was once believed to be extinct until a small remnant population was rediscovered at the type locality in *** more populations were later found on Hainan and in *** order to develop genomic resources for further studies on population genetics and conservation biology of this rare species,we identified infraspecific molecular markers in the present study,using genome skimming data of five individuals collected from two populations on Hainan Island and three populations in Vietnam *** length of plastome of the five individuals varied from 152,961 bp to 150,204 bp,and exhibited a typical angiosperm quadripartite *** plastid hotspot regions with the Pi 0.01(trnH-psbA,psbA-trnK,rpl22,ndhE,ndhG-ndhI rps15-ycf1),and 1621 polymorphic gSSRs,as well as 1657 candidate SNPs in 237 variant nuclear genes were identified,thereby providing important information for further genetic studies.