Crimped nanofiber scaffold mimicking tendon-to-bone interface for fatty-infiltrated massive rotator cuff repair

作     者：Liren Wang Tonghe Zhu Yuhao Kang Jianguang Zhang Juan Du Haihan Gao Sihao Chen Jia Jiang Jinzhong Zhao 

作者机构：Department of Sports MedicineDepartment of OrthopedicsShanghai Institute of Microsurgery on ExtremitiesShanghai Jiao Tong University Affiliated Sixth People’s HospitalNo.600 Yishan RoadShanghai200233China Regenerative Sports Medicine and Translational Youth Science and Technology Innovation WorkroomShanghai Jiao Tong University School of MedicineNo.227 South Chongqing RoadShanghai200025China Department of Medgen Group Research Laboratory18 Qinglan 3 RdShenzhen518118China Biofunctional Materials Research GroupCollege of Chemistry and Chemical EngineeringMultidisciplinary Center for Advanced MaterialsInstitute of Advanced StudiesShanghai University of Engineering ScienceNo.333 Longteng RdShanghai201620China Shanghai Jiao Tong University School of MedicineNo.227 South Chongqing RoadShanghai200025China Regenerative Sports Medicine Lab of the Institute of Microsurgery on ExtremitiesShanghai Jiao Tong University Affiliated Sixth People’HospitalNo.600 Yishan RoadShanghai200233China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第10期

页      面：149-161页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：supported by Instrumental Analysis Center of Shanghai Jiao Tong University.This work was supported by the National Natural Science Foundation of China[Grant No.81902186,81671920,31972923,81871753,81772341] National Key Research and Development Program of China[Grant No.2018YFC1106200,2018YFC1106201,2018YFC1106202] Technology Support Project of Science and Technology Commission of Shanghai Municipality of China[Grant No.19441901700,19441901701,19441901702,18441902800] 

主　　题：Massive rotator cuff tear Fatty infiltration Nanofiber scaffold Crimped structure Tendon-to-bone interface 

摘      要：Electrospun fibers,with proven ability to promote tissue regeneration,are widely being explored for rotator cuff ***,without post treatment,the microstructure of the electrospun scaffold is vastly different from that of natural extracellular matrix(ECM).Moreover,during mechanical loading,the nanofibers slip that hampers the proliferation and differentiation of migrating stem ***,electrospun nanofiber scaffolds,with crimped nanofibers and welded joints to biomimic the intricate natural microstructure of tendon-to-bone insertion,were prepared using poly(ester-urethane)urea and gelatin via electrospinning and double crosslinking by a multi-bonding network densification *** crimped nanofiber scaffold(CNS)features bionic tensile stress and induces chondrogenic differentiation,laying credible basis for in vivo *** repairing a rabbit massive rotator cuff tear using a CNS for 3 months,the continuous translational tendon-to-bone interface was fully regenerated,and fatty infiltration was simultaneously *** of micro-CT,μCT was employed to visualize the integrity and intricateness of the three-dimensional microstructure of the CNS-induced-healed tendon-to-bone interface at an ultra-high resolution of less than 1μ*** study sheds light on the correlation between nanofiber post treatment and massive rotator cuff repair and provides a general strategy for crimped nanofiber preparation and tendon-to-bone interface imaging characterization.