A melatonin-based targetable fluorescent probe for screening of tumor cells and real-time imaging of glutathione fluctuations in tumor cells

作     者：Xiwei Li Caiyun Liu Na Gao Wenlong Sheng Baocun Zhu Xiwei Li;Caiyun Liu;Na Gao;Wenlong Sheng;Baocun Zhu

作者机构：School of Water Conservancy and EnvironmentUniversity of JinanJi’nan 250022China Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Ji’nan 250103China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第5期

页      面：2527-2531页

核心收录：

学科分类：081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：support from the National Natural Science Foundation of China(Nos.21777053 and 22176070) Shandong Province Higher Educational Youth Innovation Sci-ence and Technology Program(No.2019KJD005) 

主　　题：Glutathione Reversible detection Fluorescent probe Tumor cells screening Melatonin 

摘      要：Glutathione(GSH)is a key maintainer of cellular redox balance and plays an important role in many physiological *** example,GSH has been widely implicated in cancer initiation,progression and ***,the concentrations of GSH in tumor cells can influence drug *** the serious harmfulness of cancer and the important roles of GSH in cancer,it has great significance to devel-opment probes for screening of tumor cells and real-time monitoring of GSH fluctuations in tumor ***,no targetable probe for reversible imaging of GSH in tumor cells has been ***,we constructed a melatonin-based targetable and reversible fluorescent probe(GR-MT)for screening of tumor cells and real-time imaging of GSH fluctuations in tumor *** probe uses coumarin as the skeleton,Michael addition reaction as the reaction mechanism,and melatonin as the targeted groups of tumor *** experimental results demonstrate this probe has many advantages including high selec-tivity,satisfactory sensitivity,excellent reversible ability,rapid reaction speed,and outstanding targetabil-ity of tumor ***,this study provides a promising tool for tumor cells screening and real-time detection of GSH fluctuations in specific tumor cells.