Improved salt tolerance of Populus davidiana × P. bolleana overexpressed LEA from Tamarix androssowii

作     者：Yanshuang Sun Su Chen Haijiao Huang Jing Jiang Shuang Bai Guifeng Liu 

作者机构：State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2014年第25卷第4期

页      面：813-818页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by the National High-Tech Research and Development Program of China 863 Program(Molecular Breeding and New Species Creating of Poplar)(Grant No.2011AA100201) 

主　　题：Populus davidiana x p. bolleana LEA gene transformation salt tolerance 

摘      要：Development of transgenic plants with tolerance to environ- mental stress is an important goal of plant biotechnology. （LEA） proteins accumulate in seeds dur- ing late embryogenesis, where they protect cellular membranes and macromolecules against drought. In this work, we transferred the Tamarix androssowii LEA gene into hybrids of Populus davidiana xp. bolleana. We compared relative rates of height growth, chlorophyll fluo- rescence kinetic parameters, and leaf Na＋ levels of six TaLEA-containing lines with non-transferred plants （NT）, all grown under 0.8% NaC1 stress condition. Survival percentages of transgenic lines were all higher than for NT controls after rehydration and the sur- vival percentage of SL2 was five-fold higher than for NT controls. Seed- ling height increased 48.7% in SL2 （from the onset of induced stress to the end of the growing season）, 31% more than for the NT controls. Chlorophyll fluorescence kinetic parameters showed a marked increase in photosynthetic capacity in SL2 and SL5. Na＋ levels in young leaves of transgenic lines were lower than in control NT leaves, but higher in yel- low and withered leaves, indicating improved salt tolerance in transgenic lines.