Isolation and characterization of a thermophilic Bacillus shackletonii K5 from a biotrickling filter for the production of polyhydroxybutyrate

作     者：Yong Liu Shaobin Huang Yongqing Zhang Fuqian Xu 

作者机构：Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control College of Environment and EnergySouth China University of Technology State Key Laboratory of Pulp and Paper Engineering South China University of Technology The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of Education China The Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2014年第26卷第7期

页      面：1453-1462页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No. 51378217, U1360101) the research Project of Guangdong Provincial Department of Science and Technology Department (No. 2012A010800006) the Guangdong Natural Science and Foundation (No. S2012020010887) 

主　　题：Polyhydroxyalkanotes （PHAs） Lipopolysaccharide Thermophilic Polyhydroxybutyrate Bacillus shackletonii Batch cultivation 

摘      要：Polyhydroxyalkanoates（PHAs） are aliphatic polyesters accumulated intracellularly by both Gram-negative and Gram-positive bacteria. However, compared to the PHAs of Gramnegative bacteria, few endotoxins（lipopolysaccharides, LPS）, which would be co-purified with PHAs and cause immunogenic reactions, are found in the PHAs produced by Gram-positive bacteria. A thermophilic Gram-positive bacterium K5, which exhibited good growth and polyhydroxybutyrate（PHB）-accumulating ability, has been isolated and characterized from a biotrickling filter designed for the removal of NOx from flue gas in a coal-fired power plant in China. Based on the biochemical characterization and 16 S rRNA gene sequence（Genbank accession no. JX437933）, the strain K5 has been identified as Bacillus shackletonii, which has rarely been reported in the literature, and this report is the first time that B. shackletonii has been found to accumulate PHB. The strain K5 was able to utilize glucose as carbon source to synthesize PHB at a broad range of temperatures（from 35 to 50 °C）, and the ideal temperature was 45 °C. The strain K5 could effectively yield PHB of up to 69.9% of its cell dry weight（CDW）（2.28 g/L） in flask experiments employing glucose as carbon source at 45 °C, followed by 56.8% and 52.3% of its CDW when using sodium succinate and glycerol as carbon source, respectively. For batch cultivation, the strain K5 was able to produce PHB of up to 72.6% of its cell dry weight（9.76 g/L） employing glucose as carbon source at 45 °C and pH 7.0.