Dental and periodontal phenotype in sclerostin knockout mice

作     者：Ulrike Kuchler Uwe Y Schwarze Toni Dobsak Patrick Heimel Dieter D Bosshardt Michaela Kneissel Reinhard Gruber 

作者机构：Department of Oral Surgery Medical University of Vienna Vienna Austria Austrian Cluster for Tissue Regeneration Vienna Austria Department of Oral Surgery and Stomatology School of Dental Medicine University of Berne Berne Switzerland Karl Donath Laboratory for Hard Tissue and Biomaterial Research University Clinic of Dentistry Medical University of Vienna Vienna Austria Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Research Center Vienna Austria Robert K. Schenk Laboratory of Oral Histology School of Dental Medicine University of Berne Berne Switzerland Musculoskeletal Disease Area Novartis Institutes for BioMedical Research Basel Switzerland Laboratory of Oral Cell Biology School of Dental Medicine University of Berne Berne Switzerland 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2014年第6卷第2期

页      面：70-76页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：The Department of Oral Surgery Head Professor G Watzek Bernhard Gottlieb Dental School and the Medical University of Vienna financially supported the analysis 

主　　题：alveolar bone micro-computed tomography mouse periodontium sclerostin tooth 

摘      要：Sclerostin is a Wnt signalling antagonist that controls bone metabolism. Sclerostin is expressed by osteocytes and cementocytes; however, its role in the formation of dental structures remains unclear. Here, we analysed the mandibles of sclerostin knockout mice to determine the influence of sclerostin on dental structures and dimensions using histomorphometry and micro-computed tomography （μCT） imaging, μCT and histomorphometric analyses were performed on the first lower molar and its surrounding structures in mice lacking a functional sclerostin gene and in wild-type controls, pCT on six animals in each group revealed that the dimension of the basal bone as well as the coronal and apical part of alveolar part increased in the sclerostin knockout mice. No significant differences were observed for the tooth and pulp chamber volume. Descriptive histomorphometric analyses of four wild-type and three sclerostin knockout mice demonstrated an increased width of the cementum and a concomitant moderate decrease in the periodontal space width. Taken together, these results suggest that the lack of sclerostin mainly alters the bone and cementum phenotypes rather than producing abnormalities in tooth structures such as dentin.