Single-cell RNA sequencing reveals classical monocytes are the major precursors of rat osteoclasts

作     者：JIRUI WEN WENCHAO WU MIN TANG MINGYUE BAO XUELING HE XINGHONG YAO LIANG LI 

作者机构：Institute of Biomedical EngineeringWest China School of Basic Medical Science and Forensic MedicineSichuan UniversityChengdu610041China Laboratory of Cardiovascular DiseasesRegenerative Medicine Research CenterWest China HospitalSichuan UniversityChengdu610041China Laboratory Animal CenterSichuan UniversityChengdu610041China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第3期

页      面：655-665页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.11572209,11872260) National Natural Science Foundation of China(Key Program,No.11932014) 

主　　题：Single-cell RNA sequencing Monocytes Subsets Osteoclast differentiation 

摘      要：To dissect which subset of bone marrow monocyte is the major precursor of osteoclast,3-month-old rat bone marrow was obtained for single-cell RNA sequencing.A total of 6091 cells were acquired for detailed analysis,with a median number of 1206 genes detected per cell and 17,959 genes detected in total.A total of 19 cell clusters were recognized,with the main lineages identified as B cells,Granulocytes,Monocytes,T cells,Erythrocytes and *** were further divided into classical monocytes and non-classical *** with non-classical monocytes,classical monocytes highly expressed osteoclast differentiation related genes Mitf,Spi1,Fos and ***,biological processes of classical monocytes were related to osteoclast *** revealed differentially expressed genes of classical monocytes played a role in osteoclast *** conclusion,classical monocytes were identified as the main precursors of osteoclasts in rats,and may contribute to osteoclast differentiation by regulating S100a4,S100a6,S100a10,Fn1,Vcan and *** results of this study contribute to the understanding of the origin of osteoclasts and may provide potential biomarkers for early diagnosis of bone metabolic diseases,as well as molecular and cellular targets for clinical intervention in bone metabolic diseases.