Ligninolytic enzymes production by endophytic fungus Diaporthe phaseolorum(Desm.)*** the influence of different carbon and nitrogen sources
作者机构：Mycology and Plant Pathology Lab.Department of BotanyPanjab University ChandigarhChandigarh-160014India
出 版 物：《Studies in Fungi》 (真菌研究（英文）)
年 卷 期：2021年第6卷第1期
页 面：531-542页
学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学]
基 金：Acknowledgments The authors acknowledge the Department of Botany,Panjab University Chandigarh,India,for providing infrastructure and instrumentation.Vijay Kumar is also thankful for Senior Research Fellowship(File No.09/135(0854)/2019-EMR-I) the Council of Scientific and Industrial Research(CSIR),India,during research work
主 题：Hydrolytic enzymes Industrially In vitro Manganese peroxidase Morpho molecular analysis
摘 要：Endophytic fungi are being investigated for their ability to create industrially relevant secondary *** recent years,there has been a surge in interest in these fungi as a source of novel enzymes,particularly hydrolytic *** present study investigated the effect of different carbon,organic and inorganic nitrogens on the growth and ligninolytic enzyme production by the endophytic fungus Diaporthe *** fungus was isolated from the leaves of Dillenia indica and analyzed by morpho-molecular *** fungus showed promising results for in vitro production of ligninolytic *** was the most favorable carbon compound for growth among all the carbon compounds *** displayed maximum lignin peroxidase(Lip)activity in fructose(3.5 U/ml),followed by pectin(2.60 U/ml)and glucose(2.53 U/ml).Glucose gave the highest manganese peroxidase(MnP)activity i.e.,6.88 U/ml followed by starch,sucrose and ***,the maximum laccase activity was 44.5 U/ml in *** nitrate and L-asparagine were the best inorganic and organic nitrogen for *** the case of ligninolytic enzyme production,ammonium acetate and ammonium phosphate were the best media for LiP and MnP,whereas laccase production was highest in ammonium nitrate supplemented *** organic nitrogen sources,medium supplemented with DL-tryptophan gave the highest Lip production,whereas MnP and laccase production was observed in the medium containing L-tyrosine and *** the best of our knowledge,this is the first report related to the growth and ligninolytic enzyme production by Diaporthe *** findings from the study will assist researchers in improving the production of ligninolytic enzymes by this fungus under in vitro conditions on an industrial scale.
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