Construction and characterization of a full-length infectious clone of Getah virus in vivo

作     者：Tongwei Ren Xiangling Min Qingrong Mo Yuxu Wang Hao Wang Ying Chen Kang Ouyang Weijian Huang Zuzhang Wei Tongwei Ren;Xiangling Min;Qingrong Mo;Yuxu Wang;Hao Wang;Ying Chen;Kang Ouyang;Weijian Huang;Zuzhang Wei

作者机构：Laboratory of Animal Infectious Diseases and Molecular ImmunologyCollege of Animal Science and TechnologyGuangxi UniversityNanning530005China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2022年第37卷第3期

页      面：348-357页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：funded by the Natural Science Foundation of Guangxi Province(No.2018GXNSFDA281021) the Foundation of Guangxi University(No.XGZ130959) 

主　　题：Getah virus(GETV) Reverse genetic system Expression vector 

摘      要：Getah virus(GETV)is a mosquito-borne virus of the genus Alphavirus in the family Togaviridae and,in recent years,it has caused several outbreaks in *** molecular basis for GETV pathogenicity is not well ***,a reverse genetic system of GETV is needed to produce genetically modified viruses for the study of the viral replication and its pathogenic ***,we generated a CMV-driven infectious cDNA clone based on a previously isolated GETV strain,GX201808(pGETV-GX).Transfection of pGETV-GX into BHK-21 cells resulted in the recovery of a recombinant virus(rGETV-GX)which showed similar growth characteristics to its parental *** three-day-old mice were experimentally infected with either the parental or recombinant *** recombinant virus showed milder pathogenicity than the parental virus in the *** on the established CMV-driven cDNA clone,subgenomic promoter and two restriction enzyme sites(BamHI and EcoRI)were introduced into the region between E1 protein and 3’*** the green fluorescent protein(GFP),red fluorescent protein(RFP)and improved light-oxygen-voltage(iLOV)genes were inserted into the restriction enzyme *** of the constructs carrying the reporter genes into BHK-21 cells proved the rescue of the recombinant reporter *** together,the establishment of a reverse genetic system for GETV provides a valuable tool for the study of the virus life cycle,and to aid the development of genetically engineered GETVs as vectors for foreign gene expression.