Clathrin heavy chain is essential for the development and reproduction of Locusta migratoria
作者机构：Institute of Applied BiologyShanxi UniversityTaiyuanChina Collge of Life ScienceShanxi UniversiyTaiyuanChina Institute of BiologyUniversity of SiegenSiegenGermany Department of EntomologyKansas State UniversityManhattanKansasUSA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第6期
页 面：1601-1611页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：supported by theNational Natural Science Foundation of China(NSFC31730074)
主 题：clathrin heavy chain development Locusta migratoria ovary RNA inter-ference
摘 要：Clathrin heavy chain(Chc)is a constituent of clathrin-coated vesicles and serves important functions in endocytosis and intracellular membrane trafficking but ap-pears to have physiological roles also at the organismal *** of what we know about Chc functions originates from studies performed in fungal or vertebrate ***-ever,the physiological functions of Chc in insects remain poorly ***,we identified a Chc ortholog from a Locusta migratoria transcriptome ***-qPCR revealed that LmChc was constitutively expressed in fifth-instar *** this develop-mental stage,LmChc showed the highest expression in the ovary followed by hemolymph,testis,hindgut,midgut,and *** isolated hemocytes,we detected the Chc protein in patches at the plasma *** examine the role of LmChc in *** during development,RNA interference was performed by injecting dsRNA into the early fifth-instar *** of LmChc caused a lethal phenotype with molting defect from nymph to *** addition,silencing of LmChc resulted in abnormal development of the ovaries,the size of which was significantly smaller than that in *** together,our results suggest that LmChc is a vital gene in *** that plays an important role in growth,development,and *** may be used as an efficient RNAi target gene for developing dsRNA-based biological insecticides to manage insect pests.
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