Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides

作     者：LIU Cui-qiong~1 XIA Xin-li~1 YIN Wei-lun~1 ZHOU Jiang-hong~2 TANG Hao-ru~3(1 College of Biological Sciences and Biotechnology,Beijing Forestry University 2 Phytopathology Key Laboratory,Yunnan Agricultural University 3 College of Forest and Horticulture,Sichuan Agricultural University) 

出 版 物：《北京林业大学学报》 (Journal of Beijing Forestry University)

年 卷 期：2006年第28卷第S2期

页      面：179-179页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

主　　题：plant regeneration Hippophae rhamnoides L. somatic embryogenesis 

摘      要：Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in Hippophae rhamnoides L. was achieved. The influence of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants was studied. The highest frequency of somatic embryo production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg/L kinetin (KIN) and 0.2, 0.5 mg/L indole-3-butyric acid (IAA). Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55%. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos.