Plant Growth Regulators, Light and Temperature Influenced Micropropagation and Successful Field Establishment of Vernonia amygdalina Del.

作     者：F. B. Lewu A. J. Afolayan 

作者机构：Department of Agriculture University of Zululand Kwa-Dlangezwa 3886 South Africa Department of Botany University of Fort Hare Alice 5700 South Africa 

出 版 物：《Journal of Agricultural Science and Technology(B)》 (农业科学与技术（B）)

年 卷 期：2011年第1卷第2期

页      面：216-222页

学科分类：090403[农学-农药学(可授农学、理学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 

主　　题：Eastern cape growth regulators light medicinal vegetable micropropagation temperature Vernonia amygdalina. 

摘      要：Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the winter period, thereby making large scale cultivation through conventional vegetative propagation impossible. In our effort to increasing the population of the species within the province, a micropropagation approach through tissue culture teehnology was employed. Two growth regulators （BAP ＆ NAA） were tested at four levels of concentration under continuous light and dark conditions. Plantlets were subsequently transferred to the field after acclimatization at different temperature conditions. The result of the study showed that BAP generally performs better than NAA for callus induction under continuous darkness, while direct micropropagation of shoot under continuous light condition at I mg L^-1BAP showed the best result. To achieve greater success for the rapid multiplication of the species, this study further demonstrates that the optimum temperature range for acclimatizing the species prior to the transfer of the plantlet to the field is between 23 ±3 ℃.