UPLC-QTOF/MS Method for Screening and Determination of 59 Non-labeled Components in Veterinary Drug Preparations

作     者：Xueliang PANG Lei WANG Biao QI Sining TANG Yanhua YAN Xuesong WANG Lixue DONG Yi ZHANG Aijun LI 

作者机构：Tangshan Food and Drug Comprehensive Inspection and Testing Center/Tangshan Agricultural Products Quality and Safety Inspection and Testing CenterTangshan 063000China Food Inspection Full Lab AutomationHebei Engineering Research CenterTangshan 063000China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2022年第11卷第1期

页      面：86-89,96页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Key R&D Project of Hebei Province(19227516D) Hebei Provincial Phase II Modern Agricultural Industry Technology System Innovation Team Building Project(HBCT2018120207) Hebei Provincial Phase II Modern Agricultural Industry Technology System Grass Industry Innovation Team Building Project(HBCT2018160403) Hebei Provincial Science and Technology Innovation Leading Talents(21130243A) The Fourth Batch of High-end Talent Project in Hebei Province 

主　　题：High performance liquid chromatography-tandem time-of-flight mass spectrometry Veterinary drugs Non-standard component 

摘      要：[Objectives]This study was conducted to provide an accurate and reliable method for rapid high-throughput screening of veterinary drug preparations.[Methods]For the matrixes of veterinary drug preparations,high-performance liquid chromatography-high resolution quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS)was used to establish a fast screening method for 59 non-standard components in five categories of antiviral agents,aminoglycosides,quinolones,sulfonamides,and tetracyclines in veterinary *** target drugs were separated by a Waters ACQUITY UPLC HSS T3 chromatographic column(50 mm×2.1 mm,1.8μm),and data were collected in the positive ion *** separation of the 59 drugs was achieved within 7 min.[Results]In the concentration range of 0-100 ng/ml,each drug showed a good linear relationship,and the correlation coefficients were all greater than *** detection limits of the 59 drugs were in the range of 0.1-0.5 mg/ml,and the recovery under the addition concentration of 5 mg/ml was in the range of 85.2%-103.8%.[Conclusions]The method is fast,simple,accurate,and highly sensitive,and is suitable for high-throughput screening and qualitative identification of non-standard components in veterinary drug preparations.