Seed dormancy,germination and storage behavior of Magnolia sinica,a plant species with extremely small populations of Magnoliaceae

作     者：Liang Lin Lei Cai Lei Fan Jun-Chao Ma Xiang-Yun Yang Xiao-Jian Hu Liang Lin;Lei Cai;Lei Fan;Jun-Chao Ma;Xiang-Yun Yang;Xiao-Jian Hu

作者机构：The Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small PopulationsKey Laboratory far Plant Diversity and Biogeography of East AsiaKunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2022年第44卷第1期

页      面：94-100页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the Shandong Provincial Agricultural Elite Varieties Project 'Research on key techniques for conservation of wild forest species in germplasm bank' the Biological Resources Programme, Chinese Academy of Sciences the Science & Technology Basic Resources Investigation Program of China the open research project of "Cross-Cooperative Team" of the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences 国家自然科学基金 

主　　题：Magnolia sinica Seed Dormancy Storage behavior Cryopreservation Excised embryo 

摘      要：Magnolia sinica is one of the most endangered Magnoliaceae species in China.Seed biology information concerning its long-term ex situ conservation and utilization is insufficient.This study investigated dormancy status,germination requirements and storage behavior of M.sinica.Freshly matured seeds germinated to ca.86.5%at 25/15℃but poorly at 30℃;GA3 and moist chilling promoted germination significantly at 20℃.Embryos grew at temperatures(alternating or constant)between 20℃and 25℃,but not at 5℃or 30℃.Our results indicate that M.sinica seeds possibly have non-deep simple mor-phophysiological dormancy(MPD).Seeds survived desiccation to 9.27%and 4.85%moisture content(MC)as well as a further 6-month storage at-20℃and in liquid nitrogen,including recovery in vitro as excised embryos.The established protocol ensured that at least 58%of seedlings were obtained after both cold storage and cryopreservation.These results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods,although further optimized approaches are recommended for this critically endangered magnolia species.