Effects of electroacupuncture pretreatment on M1 polarization of alveolar macrophages in rats with acute lung injury

作     者：ZHANG Yi SU Jingchao CHENG Chen WANG Caiyun MIAO Qing ZHANG Jingtao ZHANG Xinfang XIANG Shuiying LIU Zibing 张毅;苏景超;程晨;王彩云;苗青;张景涛;张新芳;项水英;刘自兵

作者机构：Graduate SchoolAnhui University of Chinese MedicineHefei 230012China Jingxian County Hospital of Traditional Chinese MedicineJingxian 242500China School of Integrated Traditional Chinese and Western MedicineAnhui University of Chinese MedicineHefei 230012China Institute of Acupuncture and MeridianCollege of Acupuncture-moxibustion and TuinaAnhui University of Chinese MedicineHefei 230012China 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学（英文版）)

年 卷 期：2022年第20卷第1期

页      面：22-31页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：国家自然科学基金项目,No.81373743 安徽省自然科学基金项目,No.2008085MH267 安徽省省级大学生创新训练项目,No.202006170243 

主　　题：Acupuncture Therapy Electroacupuncture Acute Lung Injury Macrophages Alveolar Tumor Necrosis Factor-alpha NF-kappa B Signal Transduction Rats 

摘      要：Objective:To observe the effects of electroacupuncture(EA)pretreatment on M1 polarization of alveolar macrophages(AMs)in rats with acute lung injury(ALI)induced by lipopolysaccharide(LPS),and to explore the potential protective mechanism of ***:Forty Sprague-Dawley rats were randomly divided into a normal group,a model group,and three groups of EA pretreatment[including a Chize(LU5)group,a Zusanli(ST36)group and a Chize(LU5)plus Zusanli(ST36)group],with eight rats in each *** model rats of ALI were established by instilling LPS[2 mg/(kg·bw)]into the trachea of rats for 3 *** rats in each EA pretreatment group were pretreated with EA for 30 min per day at the corresponding bilateral acupoints 6 d before instilling *** hours after modeling,the pulmonary function of the rats was tested,and the lung tissue was taken to calculate the ratio of lung wet weight to dry weight(W/D).The pathological lung changes and the injury score were observed by hematoxylin-eosin *** contents of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,and myeloperoxidase(MPO)in rat s bronchoalveolar lavage fluid(BALF)were detected by enzyme-linked immunosorbent *** mRNA and protein expression levels of M1 macrophage markers clusters of differentiation 86(CD86),inducible nitric oxide synthase(iNOS),and its signaling pathway factor Toll-like receptor(TLR)4,and nuclear factor-κB(NF-κB)p65 in the alveoli were detected by fluorescence quantitative polymerase chain reaction and Western blot,***:After being induced by LPS,the pulmonary function of the model rats showed that the forced expiratory volume in 0.1 s(FEV0.1),forced expiratory volume in 0.3 s(FEV0.3),and their respective ratios of FEV to forced vital capacity(FVC)(including FEV0.1/FVC and FEV0.3/FVC)were significantly decreased(P0.01),while the W/D of lung tissue was increased(P0.01).The score of lung injury was significantly higher(P0.01).The contents of TNF-α,IL-1β,and MPO in the BALF and