Bradykinin postconditioning protects rat hippocampal neurons after restoration of spontaneous circulation following cardiac arrest via activation of the AMPK/mTOR signaling pathway

作     者：Shi-Rong Lin Qing-Ming Lin Yu-Jia Lin Xin Qian Xiao-Ping Wang Zheng Gong Feng Chen Bin Song Shi-Rong Lin;Qing-Ming Lin;Yu-Jia Lin;Xin Qian;Xiao-Ping Wang;Zheng Gong;Feng Chen;Bin Song

作者机构：Provincial College of Clinical MedicineFujian Medical UniversityFuzhouFujian ProvinceChina Department of EmergencyFujian Provincial Hospital South BranchFuzhouFujian ProvinceChina Department of EmergencyFujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Emergency Medical CenterFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Emergency MedicineFuzhouFujian ProvinceChina Department of Human AnatomySchool of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian ProvinceFuzhouFujian ProvinceChina Laboratory of Clinical Applied AnatomyFujian Medical UniversityFuzhouFujian ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第10期

页      面：2232-2237页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the Fujian Provincial Health Technology Project of China,No.2018-CX-16 Fujian Provincial Hospital Flint Fund Project,No.2020HSJJ17(both to SRL) 

主　　题：autophagy bradykinin cardiac arrest cardiopulmonary resuscitation compound C hippocampus neuron rapamycin restoration of spontaneous circulation 

摘      要：Bradykinin(BK)is an active component of the kallikrein-kinin system that has been shown to have cardioprotective and neuroprotective *** previously showed that BK postconditioning strongly protects rat hippocampal neurons upon restoration of spontaneous circulation(ROSC)after cardiac ***,the precise mechanism underlying this process remains poorly *** this study,we treated a rat model of ROSC after cardiac arrest(induced by asphyxiation)with 150μg/kg BK via intraperitoneal injection 48 hours after ROSC following cardiac *** found that BK postconditioning effectively promoted the recovery of rat neurological function after ROSC following cardiac arrest,increased the amount of autophagosomes in the hippocampal tissue,inhibited neuronal cell apoptosis,up-regulated the expression of autophagy-related proteins LC3 and NBR1 and down-regulated p62,inhibited the expression of the brain injury marker S100βand apoptosis-related protein caspase-3,and affected the expression of adenosine monophosphate-activated protein kinase/mechanistic target of rapamycin pathway-related *** monophosphate-activated protein kinase inhibitor compound C clearly inhibited BK-mediated activation of autophagy in rats after ROSC following cardiac arrest,which aggravated the injury caused by *** mechanistic target of rapamycin inhibitor rapamycin enhanced the protective effects of BK by stimulating *** findings suggest that BK postconditioning protects against injury caused by ROSC through activating the adenosine monophosphate-activated protein kinase/mechanistic target of the rapamycin pathway.