Identification of reference genes provides functional insights into meiotic recombination suppressors in Gerbera hybrida

作     者：Fan Li Ying Cheng Lulin Ma Shenchong Li Jihua Wang Fan Li;Ying Cheng;Lulin Ma;Shenchong Li;Jihua Wang

作者机构：Floriculture Research InstituteYunnan Academy of Agricultural SciencesNational Engineering Research Center for Ornamental HorticultureKey Laboratory for Flower Breeding of Yunnan ProvinceKunming 650200China School of AgricultureYunnan UniversityKunming 650504China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2022年第8卷第1期

页      面：123-132页

核心收录：

学科分类：0710[理学-生物学] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 0902[农学-园艺学] 

基　　金：funded by Yunnan Fundamental Research Projects(Grant No.2019FD030) Major Science and Technology Project of Yunnan Provincial Department of Science and Technology(Grant Nos.2019ZG006,202102AE090052) Ten-thousand Talents Program of Yunnan Province–Yunling Scholar of Industrial Technology Leading Talent Project(Grant No.Yun Fagai RenshiNo.212)。 

主　　题：erbera hybrida Reference gene Gene expression Meiotic recombination suppressor 

摘      要：Gerbera Hybrida is one of the important cut flowers across the world.The novel traits are the primarily market requirements and the breeding targets,mainly determined by the degree of genetic variation after hybridization.However,meiotic recombination is highly conserved in most eukaryotes which suppressed the crossover formation and limited the genetic diversity.Recently,several meiotic recombination suppressors have been identified and characterized in plants,whereas it remains elusive in G.hybrida.In order to characterize the expression patterns of these suppressors in G.hybrida,20 candidate reference genes were identified from the transcriptome datasets of G.hybrida,and their expression stabilities during plant development were evaluated by geNorm,NormFinder and BestKeeper.Although the most stable reference genes were variable in different softwares,comprehensive ranking revealed that PGK2 was the most stable reference gene and GAPDH was the most unstable one.The expression patterns of FANCM,FIGL1,RECQ4,RM1,and FLIP further validated that PGK2 was suitable for normalization of gene expression.Our study identified a reliable reference gene for gene expression during meiotic recombination,and provided functional insights into meiotic recombination suppressors in G.hybrida.