Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time
Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time作者机构：State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Syngenta Biotechnology China Co. Ltd. Biotechnology Research Institute Chinese Academy of Agricultural Sciences
出 版 物：《National Science Review》 (国家科学评论(英文版))
年 卷 期：2022年第9卷第1期
页 面：56-65页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 0703[理学-化学]
基 金：supported by the National Natural Science Foundation of China (31370308 and 31921001) the China Postdoctoral Science Foundation (2018M641533) the Chinese Universities Scientific Fund (2019TC122 and 2019TC228)
主 题：calcium signaling CPK32 FCA FLC flowering time
摘 要：Appropriate flowering time is critical for the reproductive success of plant species.Emerging evidence indicates that calcium may play an important role in the regulation of flowering time.However,the underlying molecular mechanisms remain unclear.In this study,we demonstrate that calcium-dependent protein kinase 32(CPK32) regulates flowering time by affecting the alternative polyadenylation of FLOWERING CONTROL LOCUS A(FCA) and altering the transcription of FLO WERING LOCUS C(FLC),a central repressor of flowering time.The knockdown of CPK32 results in an obvious late flowering phenotype and dramatically enhanced FL C transcription.CPK32 interacts with FCA,and phosphorylates the serine592of FCA in a Ca2+-dependent manner.Moreover,the ratio of abundance of the FCA transcripts(FCA-D and FCA-P) changes significantly in the cpk32 mutant,which subsequently affects FLC expression and consequently regulates floral transition.The present evidence demonstrates that CPK32 modulates flowering time by regulating FCA alternative polyadenylation and consequent FL C expression.
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