Effects of red orpiment on cell morphology and expression of PML mRNA and protein in NB4 and HL-60 cells

作     者：钟璐 陈芳源 韩洁英 邵念贤 欧阳仁荣 

作者机构：Department of Leukemia Research 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第1期

页      面：148-150页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：ThisstudywaspartiallysupportedbytheNationalNaturalScienceFoundationofChina (No 36 970 32 8) 

主　　题：red orpiment NB4 cell HL-60 cell PML protein 

摘      要：Objective To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell *** Cell morphology was assayed by Wright s staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining. Results The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated *** Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.