Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in latephase laying hens

作     者：Jia Feng Mingyuan Lu Jing Wang Haijun Zhang Kai Qiu Guanghai Qi Shugeng Wu Jia Feng;Mingyuan Lu;Jing Wang;Haijun Zhang;Kai Qiu;Guanghai Qi;Shugeng Wu

作者机构：Laboratory of Quality&Safety Risk Assessment for Animal Products on Feed Hazards(Beijing)of the Ministry of Agriculture&Rural AffairsFeed Research InstituteChinese Academy of Agricultural SciencesNo.12 Zhongguancun South St.Haidian DistrictBeijing 10081China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第1期

页      面：265-279页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by Shandong Key Science and Technology Innovation Program(2019JZZY010704) China Agriculture Research System(CARS-40-K12) Beijing Innovation Consortium of Agriculture Research System(BAIC04-2020) Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences. 

主　　题：Essential oil Feed efficiency Immune status Late-phase laying hen Microbiota 

摘      要：Background:Dietary essential oil(EO)supplementation can exert favorable effects on gut health in broilers.However,it is unknown whether EO could improve intestinal functions,consequently beneficial for egg performance and quality in late-phase laying hens.This study was aimed to investigate the potential effects of EO on production performance,egg quality,intestinal health and ileal microbiota of hens in the late phase of production.A total of 28860-week-old Hy-line Brown laying hens were randomly divided into 4 groups and fed a basal diet(control)or basal diets supplemented with oregano EO at 100,200 and 400 mg/kg(EO100,EO200 and EO400).Results:Dietary EO supplementation resulted in a quadratic decrease(P0.05)in feed conversion ratio with lower(P0.05)feed conversion ratio in EO200 group than the control during weeks 9–12 and 1–12 of the trial.Compared to the control,EO addition resulted in higher(P0.05)eggshell thickness at the end of week.4,8 and 12 and higher(P0.05)chymotrypsin activity.There was a quadratic elevation(P0.05)in ileal chymotrypsin and lipase activity,along with a linear increase in villus height to crypt depth ratio.Quadratic declines(P0.05)in mRNA expression of IL-1β,TNF-α,IFN-γ and TLR-4,concurrent with a linear and quadratic increase(P0.05)in ZO-1 expression were identified in the ileum with EO addition.These favorable effects were maximized at medium dosage(200 mg/kg)of EO addition and intestinal microbial composition in the control and EO200 groups were assessed.Dietary EO addition increased(P0.05)the abundances of Burkholderiales,Actinobacteria,Bifidobacteriales,Enterococcaceae and Bacillaceae,whereas decreased Shigella abundance in the ileum.Conclusions:Dietary EO addition could enhance digestive enzyme activity,improve gut morphology,epithelial barrier functions and modulate mucosal immune status by altering microbial composition,thus favoring feed efficiency and eggshell quality of late-phase laying hens.