3D Bioprinted Spheroidal Droplets for Engineering the Heterocellular Coupling between Cardiomyocytes and Cardiac Fibroblasts
作者机构：Inspired Materials&Stem-Cell Based Tissue Engineering Laboratory(IMSTEL)The University of Texas at El PasoEl PasoTX 79968USA Department of MetallurgicalMaterialsand Biomedical EngineeringM201 EngineeringThe University of Texas at El Paso500 W.University AvenueEl PasoTX 79968USA Department of Molecular and Translational MedicineCenter of Emphasis in Diabetes and MetabolismTexas Tech University Health Sciences Center5001 El Paso DriveEl PasoTX 79905USA Border Biomedical Research CenterThe University of Texas at El Paso500 W.University AvenueEl PasoTX 79968USA
出 版 物：《Cyborg and Bionic Systems》 (类生命系统（英文）)
年 卷 期：2021年第2021卷第1期
页 面：206-221页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0811[工学-控制科学与工程] 0812[工学-计算机科学与技术（可授工学、理学学位）] 10[医学]
基 金：support from National Institute of General Medical Sciences of the National Institutes of Health under RL5GM118969 TL4GM118971 the NIH BUILD Pilot grant#8UL1GM118970-02
摘 要：Since conventional human cardiac two-dimensional(2D)cell culture and multilayered three-dimensional(3D)models fail in recapitulating cellular complexity and possess inferior translational capacity,we designed and developed a high-throughput scalable 3D bioprinted cardiac spheroidal droplet-organoid model with cardiomyocytes and cardiac fibroblasts that can be used for drug screening or regenerative engineering *** study helped establish the parameters for bioprinting and cross-linking a gelatin-alginate-based bioink into 3D spheroidal droplets.A flattened disk-like structure developed in prior studies from our laboratory was used as a *** microstructural and mechanical stability of the 3D spheroidal droplets was assessed and was found to be ideal for a cardiac *** human cardiac fibroblasts and AC16 cardiomyocytes were mixed in the bioink and ***-dead assay and flow cytometry analysis revealed robust biocompatibility of the 3D spheroidal droplets that supported the growth and proliferation of the cardiac cells in the long-term ***,the heterocellular gap junctional coupling between the cardiomyocytes and cardiac fibroblasts further validated the 3D cardiac spheroidal droplet model.
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