Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production
作者机构：Department of BiotechnologyDelft University of TechnologyVan der Maasweg 92629HZ Delftthe Netherlands DSM Biotechnology CentreAlexander Fleminglaan 12613AX Delftthe Netherlands
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2022年第7卷第1期
页 面：554-566页
核心收录：
学科分类：08[工学] 0836[工学-生物工程] 0822[工学-轻工技术与工程] 082203[工学-发酵工程]
基 金：DSM Bio-based Products & Services B.V.
主 题：Saccharomyces cerevisiae Synthetic biology Metabolic engineering Biofuels Redox metabolism Energy metabolism
摘 要：Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae.This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production,yeast growth and glycerol formation.Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen meta-bolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism.In addition to providing an overview of scientific advances we discuss context dependency,theoretical impact and potential for industrial application of different proposed and developed strategies.
维普期刊数据库