Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes

作     者：Tao-Rong Xie Chun-Feng Liu Jian-Sheng Kang 

作者机构：CAS Key Laboratory of Nutrition and MetabolismInstitute for Nutritional SciencesShanghai Institutes for Biological SciencesGraduate School of the Chinese Academy of SciencesChinese Academy of Sciences 

出 版 物：《Biophysics Reports》 (生物物理学报)

年 卷 期：2017年第3卷第Z2期

页      面：85-91页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：supported by National Basic Research Program of China (2011CB910903 and 2010CB912001) National Natural Science Foundation of China (31171369) Chinese Academy of Sciences (Hundred Talents Program and 2009OHTP10) 

主　　题：Brown adipocytes Mitochondrial thermometry Nanothermometry Thermogenesis 

摘      要：Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of temperature increase in brown adipocytes.