Research and Development of Butterfly Microfluidic Gene Chip for 19 Common Pathogenic Microorganisms of Nosocomial Infection

作     者：Jingjing Yu Xu Zhang Jinhai Tian Rong Ma Qi Huang Jia Cao Jia Wang Libin Wang 

作者机构：The General Hospital of Ningxia Medical UniversityDepartment of Beijing National Biochip Research Center sub-center in NingxiaYinchuan 750004Ningxia Hui Autonomous RegionChina 

出 版 物：《Journal of Clinical and Nursing Research》 (临床护理研究（英文）)

年 卷 期：2022年第6卷第1期

页      面：80-85页

学科分类：08[工学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：Key Research and Development Plan Project of Ningxia Hui Autonomous Region(Project No.:2019BEG03026) Ningxia Overseas Returnees Innovation and Entrepreneurship Project(Project No.:2017-659) Ningxia Key Research and Development Plan Project(Project No.2018BFG02017) Ningxia Medical University Young Backbone Talents Training Program School-level Academic Technology Leader Reserve Cultivation Object First-Class Discipline Construction Founded Project of Ningxia Medical University and the School of Clinical Medicine(No.NXYLXK2017A05) 

主　　题：Nosocomial infection Butterfly microfluidic gene chip High throughput 

摘      要：Based on butterfly microfluidic gene chip technology,a method for rapid,accurate and efficient detection of 19 common pathogenic microorganisms of nosocomial infection was established,and a butterfly microfluidic gene chip with high-throughput detection was designed and *** constant temperature amplification technology,using the polymerase with chain replacement function to react at constant temperature(65℃)and combined with microfluidic chip technology,primers were designed according to the target genes of 19 pathogenic microorganisms,and a butterfly microfluidic gene chip which can detect 19 common pathogenic microorganisms of nosocomial infection was made to simplify the inspection operation process and verify the sensitivity of the *** butterfly microfluidic gene chip can be used for the rapid and efficient detection of 19 common pathogenic microorganisms of nosocomial infection,and provides a new idea for the detection and auxiliary diagnosis of pathogenic microorganisms of nosocomial infection.