Based on network pharmacology to explore the mechanism of nephrotoxicity of Xanthii Fructus

作     者：Tao Chen Tengda Li Wenna Zhang Yulin Song Yuman Tang Rui Gong Hua Jin 

作者机构：Nankai University HospitalTianjin300071China Tianjin University of Traditional Chinese MedicineTianjin301617China Chinese Medicine CollegeTianjin University of Traditional Chinese MedicineTianjin301617China 

出 版 物：《Asian Toxicology Research》 

年 卷 期：2022年第4卷第1期

页      面：24-31页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Network pharmacology Nephrotoxicity Inflammatory responses Apoptosis 

摘      要：Objective:To explore the potential mechanism of nephrotoxicity induced by Xanthii Fructus(XF)through network ***:The active ingredients and targets of XF were screened through Traditional Chinese Medicine Systems Pharmacology Database,PubChem Database and Swiss Target Prediction Database,and the related pathogenic genes of nephrotoxicity were downloaded from Genecards,pharmGKB and OMIM *** potential target is the intersection of XF and the pathogenic gene of *** software was used to construct a network of“XF-Potential active ingredient-Target-Nephrotoxicity network.Protein protein interaction(PPI)network was constructed by String database,and the data was analyzed and sorted out to obtain the key active components and core target proteins of *** functional enrichment analysis and KEGG pathway enrichment analysis of key target proteins were performed by R ***:11 active components,167 targets,6839 nephrotoxicity related pathogenic genes and 139 intersection target genes were screened from *** constructing the network of“XF-Potential active ingredient-target-nephrotoxicity networkand PPI network,the key active ingredients of XF were identified as Moupinamide,Beta-sitosterol and Stigmasterol,and the core target proteins were CASP3,HSP90AA1 and *** were 2072 GO entries(P0.05),including 1876 Biological Process(BP),64 Cellular Component(CC)and 132 Molecular Function(MF).KEGG enrichment analysis revealed 138 pathways(P0.05),mainly involving p53 signaling pathway,IL-17 signaling pathway,PI3K-Akt signaling pathway were strongly correlated with the nephrotoxicity of ***:The core target protein of XF HSP90AA1,CASP3 and TP53 participate in p53 signaling Pathway,PI3K-Akt signaling Pathway,IL-17 signaling Pathway and other pathways to regulate apoptosis and induce inflammatory responses,resulting in kidney damage.