Mechanisms of Compound Kushen Injection for the treatment of bladder cancer based on bioinformatics and network pharmacology with experimental validation

作     者：ZHANG Li-Hui ZHANG Wan-Ying XIONG Jia-Ming DUAN Xiu-Mei HAI Li-Na ZHANG Yu-Liang ZHANG Miao-Miao QIN Gui-Fang ZHANG Guo-Wei ZHANG Li-Hui;ZHANG Wan-Ying;XIONG Jia-Ming;DUAN Xiu-Mei;HAI Li-Na;ZHANG Yu-Liang;ZHANG Miao-Miao;QIN Gui-Fang;ZHANG Guo-Wei

作者机构：College of Traditional Chinese MedicineHebei UniversityBaoding 071002China Beijing Zhendong Guangming Pharmaceutical Research InstituteBeijing 100085China Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2022年第20卷第1期

页      面：43-53页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the Training Pro-gram for Outstanding Clinical Medical Talents funded by the govern-ment(2020) the Outstanding Youth Scientific Research and Innova-tion Team(Science and Technology)Project of Hebei University(2020-8) the Medical Science Foundation of Hebei University(No.2020A15) the National Natural Science Foundation of Hebei Province(No.H2018201179) the Health and Family Planning Com-mission of Hebei Province(No.20190948) Hebei University Re-search Project of Science and Technology(No.QN2019146) 

主　　题：Compound Kushen Injection Bladder cancer Network pharmacology GEO Molecular mechanism 

摘      要：Bladder cancer is the most common malignancy of the urinary *** Kushen Injection(CKI)is a Chinese medicinal preparation that has been widely used in the treatment of various types of cancers in the past two ***,the pharmacological effect of CKI on bladder cancer is not still completely *** the current study,network pharmacology com-bined with bioinformatics was used to elucidate the therapeutic mechanism and potential targets of CKI in bladder *** mech-anism by which CKI was effective against bladder cancer was further verified in vitro using human bladder cancer cell line ***-work pharmacology analysis identified 35 active compounds and 268 target genes of *** data indicated 5500 differen-tially expressed genes associated with bladder *** genes of CKI and bladder cancer suggested that CKI exerted anti-blad-der cancer effects by regulating genes such as MMP-9,JUN,EGFR,and *** enrichment analysis indicated that CKI ex-erted therapeutic effects on bladder cancer by regulating certain biological processes,including cell proliferation,cell migration,and cell *** addition,Kyoto Encyclopedia of Genes and Genomes enrichment analysis implicated pathways related to cancer,bladder cancer,and the PI3K-Akt signaling ***,cell experiments indicated that CKI inhibited the proliferation and migration of T24 cells,and induced their ***,RT-qPCR and Western blot results demonstrated that CKI was likely to treat bladder cancer by down-regulating the gene and protein expression of MMP-9,JUN,EGFR,and *** inhibited the prolif-eration and migration,and induced the apoptosis of T24 bladder cancer cells through multiple biological pathways and *** also exhibited significant effects on the regulation of key genes and proteins associated with bladder ***,our findings provide solid evidence and deepen current understanding of the therapeutic effects