A network pharmacology-based strategy for predicting the protect-ive mechanism of Ginkgo biloba on damaged retinal ganglion cells

作     者：YU Hua DONG Li-Hong ZHANG Yan LIU Qiang YU Hua;DONG Li-Hong;ZHANG Yan;LIU Qiang

作者机构：Chaohu Hospital Affiliated to Anhui Medical UniversityHefei 230000China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2022年第20卷第1期

页      面：54-66页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Ginkgo biloba Oxidative stress Retinal ganglion cells Apoptosis p53 Acetylation 

摘      要：Hallmarks of the pathophysiology of glaucoma are oxidative stress and apoptotic death of retinal ganglion cells(RGCs).Ginkgo biloba extract(EGb)with multi-target,multi-pathway functions has been reported to exert positive pharmacological effects on oxidative stress and damaged ***,the ingredients and anti-apoptotic targets of EGb in the treatment of open-angle glaucoma(OAG)have not been fully ***,in-depth analysis is necessary for further *** biloba-re-lated and anti-apoptotic targets were identified and then combined to obtain the intersection,representing the potential anti-apoptotic targets of Ginkgo *** addition,compound-anti-apoptotic target and OAG-target protein-protein interaction network were merged to obtain five core genes and compound-OAG-anti-apoptotic target protein-protein interaction ***,the active com-pounds and anti-apoptotic targets of Ginkgo biloba in the treatment of OAG were identified,namely luteolin,β-sitosterol,kaempferol,stigmasterol,quercetin,and p53,Bax,Bcl-2,Caspase-3 and Caspase-9,*** the anti-apoptotic targets of Ginkgo biloba in the treatment of OAG,Gene Ontology(GO)and pathway analysis were executed to confirm the gene functions of Ginkgo biloba in antagonizing apoptosis of *** pathway enrichment was mainly involved in transcriptional activation of p53 responsive genes,activation of caspases and apoptotic ***,we confirmed the results of the network analysis by H2O2 treated RGC-5 cells in *** results demonstrated that EGb protection can effectively diminish H2O2-induced apoptosis by inhibiting p53 acetylation,reducing the ratio of Bax/Bcl-2 and suppressing the expression of specific cleavage of Caspase-9 and Caspase-3.