β-Elemene induces apoptosis and autophagy in colorectal cancer cells through regulating the ROS/AMPK/mTOR pathway

作     者：WANG Guo-Yu ZHANG Lei GENG Ya-Di WANG Bin FENG Xiao-Jun CHEN Zhao-Lin WEI Wei JIANG Ling WANG Guo-Yu;ZHANG Lei;GENG Ya-Di;WANG Bin;FENG Xiao-Jun;CHEN Zhao-Lin;WEI Wei;JIANG Ling

作者机构：Department of Pharmacythe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui230001China Institute of Clinical PharmacologyAnhui Medical UniversityHefei 230001China Department of PharmacyHefei Cancer HospitalChinese Academy of SciencesHefei 230031China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2022年第20卷第1期

页      面：9-21页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(Nos.81603339 and 81803774) Anhui Key Research and Development Program(No.201904a-07020092) the Fundamental Research Funds for the Central Universities(Nos.WK9110000016 and WK9110000079) China Postdoctoral Science Foundation(No.2019M662207) 

主　　题：β-Elemene Colorectal cancer Apoptosis Autophagy AMPK mTOR 

摘      要：β-Elemene is an effective anti-cancer ingredient extracted from the genus Curcuma(Zingiberaceae familiy).In the present study,we demonstrated thatβ-elemene inhibited the proliferation of colorectal cancer cells and induced cell cycle arrest in the G2/M *** addition,β-elemene induced nuclear chromatin condensation and cell membrane phosphatidylserine eversion,decreased cell mitochondrial membrane potential,and promoted the cleavage of caspase-3,caspase-9 and PARP proteins,indicating apoptosis in colorectal cancer *** the same time,β-elemene induced autophagy response,and the treated cells showed autophagic vesicle bilayer membrane structure,which was accompanied by up-regulation of the expression of LC3B and ***,β-elemene increased ROS levels in colorectal cancer cells,promoted phosphorylation of AMPK protein,and inhibited mTOR protein *** the experiments in vivo,β-elemene inhibited the tumor size and induced apoptosis and autophagy in nude *** summary,β-elemene inhibited the occurrence and development of colon cancer xenografts in nude mice,and significantly induced apoptosis and autophagy in colorectal cancer cells in *** effects were associated with regulation of the ROS/AMPK/mTOR *** offered a molecular basis for the development ofβ-elemene as a promising anti-tumor drug candidate for colorectal cancer.