Novel strategies for the biocontrol of noctuid pests(Lepidoptera)based on improving ascovirus infectivity using Bacillus thuringiensis
作者机构：Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect PestsHunan Agricultural UniversityChangshaHunan410128China College of Plant ProtectionHunan Agricultural UniversityChangshaHunan410128China Department of EntomologyUniversity of GeorgiaAthensGA30602USA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2021年第28卷第5期
页 面：1452-1467页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：the National Natural Science Foundation of China(31700141,31872027) Provincial Natural Science Foundation of Hunan(2019JJ50234) Changsha Science and Technology Project(kq 1901033) Double firstclass construction project of Hunan Agricultural University
主 题：ascovirus Bacillus thuringiensis formulation Heliothis virescens ascovirus 3h(HvAV-3h) Heliothis virescens ascovirus 3j(HvAV-3j) per os infectivity
摘 要：ldentifying novel biocontrol agents and developing new strategies are urgent goals in insect pest *** are potential competent insect viruses that may be developed into bioinsecticides,but this aim is impeded by their poor oral *** improve the per os infectivity of ascovirus,Bacillus thuringiensis kurstaki(Btk)was employed as a helper to damage the midgut of lepidopteran larvae(Helicoverpa armigera,Mythimna separata,Spodoptera frugiperda,and ***)in formulations with Heliothis virescens ascovirus isolates(HvAV-3h and HvAV-3j).Btk and ascovirus mixtures(Btk/HvAV-3h and B1k/HvAV-3j)were fed to insect larvae(3rd instar).With the exception of *** larvae,which exhibited low mortality after ingesting Btk,the larvae of the other tested species showed three types of response to feeding on the formulas:type I,the tested larvae(***)were killed by Btk infection so quickly that insufficient time and resources remained for ascoviral invasion;type II,both Btk and the ascovirus were depleted by their competition,such that neither was successfully released or colonized the tissue;type II,Btk was eliminated by the ascovirus,and the ascovirus achieved systemic infection in the tested *** feeding of Btk/ascovirus formulas led to a great reduction in larval diet consumption and resulted in a significant decrease in the emergence rate of ***,***,and *** larvae,which suggested that the formulas exerted marked oral control effects on both the contemporary individuals and the next generation of these tested pest species.
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