Involvement of chemosensory proteins in host plant searching in the bird cherry-oat aphid
作者机构：State Key Laboratory of Crop Stress Biology for Arid AreasKey Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural AffairsNorthwest A&F UniversityYang lingShaanxi Province712100China IGEPP UMR 1349INRAAgrocampus OuestUniv Rennes 1F-35653 Domaine De La MotteLe RheuFrance Shandong Academy of Agricultural SciencesShandong Peanut Research InstituteQingdaoShandong266100China
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2021年第28卷第5期
页 面：1338-1353页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：the National Natural Science Foundation of China(grant nos.31972263,31901878 and 31772160) China Postdoctoral Science Foundation(grant no.2019M653773)。
主 题：host choice experiment olfactometer Rhopalosiphum padi RNAi starvation wing form
摘 要：Chemosensory systems are considered to play an important role in host plant selection in herbivorous insects.However,few studies have focused on chemosensory proteins(CSPs)for aphid host-location mechanisms.The roles of CSPs in searching for different Poaceae species(wheat,barley,triticale,maize and sorghum)were tested in Rhopalosiphum padi,an important cereal pest.The olfactometer assays showed that R.padi responds to plant odors.Seven R.padi CSP genes were identified.Influence of aphid morph,tissue and starvation state on expression patterns of CSPs was evaluated.Expression levels of CSPI,CSP4,CSP5 and CSP6 in winged aphids were significantly higher than those in wingless ones.Transcription levels of four genes(CSPI,CSP4,CSP5 and CSP6)were relatively higher in the head with antennae,and the four genes tended to be upregulated follow ing starvation.Silencing of three CSPs(CSP4,CSP5 and CSP6)altered aphid host-location behavior in response to the five different host plants tested.Three volatile compounds of host plants(octanal,[E]-2-hexenol and linalool)have signif-icant attraction to winged R.padi according to the four-arm olfactometer tests.Molecular docking predicted hydrogen bonding sites which played key roles in the binding of CSP4,CSP5 and CSP6 with volatile compounds.Knockdown of CSP4 or CSP5 significantly decreased the staying time of R.padi in the arms with octanal.However,knockdown of CSP6 could not affect the response of R.padi to octanal.These results bring evidence for the involvement of three CSPs in R.padi host-location behavior.
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