Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy
作者机构：John Hardesty Department of OphthalmologyWashington UniversitySt.LouisMO 63110USA Department of MedicineDivision of RheumatologyWashington UniversitySt.LouisMO 63110USA Department of Ophthalmology and Visual SciencesUniversity of Maryland School of MedicineBaltimoreMD 21201USA
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2022年第15卷第1期
页 面：89-97页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by an unrestricted grant to Washington University by Research to Prevent Blindness Inc. New York NY
主 题：cell-derived microparticles vitrectomy vitreous diabetes mellitus apoptosis
摘 要：AIM:To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing ***:Un-masked cross-sectional series of 34 eyes undergoing *** specimens were collected and processed to evaluate for membrane integrity(DAPI),apoptosis(Annexin-V),and endothelial-cell origin(V-Cadherin).A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size ***:Thirty-four specimens underwent *** levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous(n=12)compared to those without blood(n=22;52.3%±30.7%vs 19.6%±27.2%,P=0.002).Patients with diabetes having surgery with hemorrhage(n=7)had greater expression of Annexin-V than those without hemorrhage(n=8;62.1%±31.7%vs 18.9%±20.9%,P=0.009).However,in patients with non-diabetic vitreous hemorrhage,the level of Annexin-V expression was not significantly different compared to other disease processes(38.6%±25.7%,n=5 vs 20.0%±30.9%,n=14,P=0.087).CONCLUSION:Increased expression of the apoptotic marker,Annexin-V is detected on vitreous microparticles in diabetes-related vitreous *** evaluating vitreous hemorrhage in patients without diabetes,the apoptotic signal is not significantly *** in patients with diabetes,and improvement in visual outcomes,may be related to the removal of a serumderived,pro-apoptotic *** investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease.
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