A Network Pharmacology-Based Study on Antidepressant Effect of Salicornia europaea *** with Experimental Support in Chronic Unpredictable Mild Stress Model Mice

作     者：SUN Dan-chen WANG Ran-ran XU Hao ZHU Xue-hui SUN Yan QIAO Shi-qing QIAO Wei 

作者机构：Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and DiagnosticsSchool of PharmacyTianjin Medical UniversityTianjin300070China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2022年第28卷第4期

页      面：339-348页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Nature Science Foundation of China(No.81173530) Tianjin Research Program of Applied Basic and Cutting-edge Technologies(No.17JCZDJC33200 and No.12JCZDJC25900) 

主　　题：Salicornia europaea L. network pharmacology tail suspension test forced swim test depression 

摘      要：Objective:To investigate the pharmacodynamic material basis,mechanism of actions and targeted diseases of Salicornia europaea L.(SE)based on the network pharmacology method,and to verify the antidepressant-like effect of the SE extract by pharmacological ***:Retrieval tools including Chinese medicine(CM),PubMed,PharmMapper,MAS 3.0 and Cytoscape were used to search the components of SE,predict its targets and related therapeutic diseases,and construct theComponent-TargetPathwaynetwork of SE for central nervous system(CNS)***,protein-protein interaction(PPI)network,Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)function annotation of depression-related targets were analyzed to predict the antidepressant mechanism of *** unpredictable mild stress(CUMS)model was used to construct a mouse model with depression-like *** the animals were randomly divided into 6 groups(n=10)including the normal group(nonstressed mice administered with distilled water),the CUMS group(CUMS mice administered with distilled water),the venlafaxine group(CUMS mice administered with venlafaxine 9.38 mg/kg),SE high-,medium-,and low-dose groups(CUMS mice administered with SE 1.8,1.35 and 0.9 g/kg,respectively).Then some relevant indicators were determined for experimental verification by the forced swim test(FST),the tail suspension test(TST)and open-field test(OFT).Dopamine(DA)concentration in hippocampus and cerebral cortex,IL-2 and corticosterone(CORT)levels in blood,and nuclear factor E2 related factor 2(Nrf2),kelch-like epichlorohydrin related protein 1(Keap1),NAD(P)H dehydrogenase[quinone]1(NQO1)and heme oxygenase-1(HO-1)levels in mice were measured by enzyme linked immunosorbent assay(ELISA)and Western blot respectively to explore the possible ***:Thetarget-diseasenetwork diagram predicted by network pharmacology,showed that the potential target of SE involves a variety of CNS diseases,among which depression ac