Proteomic analysis of pathogen-responsive proteins from maize stem apoplast triggered by Fusarium verticillioides

作     者：Hafiz ABDUL HASEEB ZHANG Jun GUO Yu-shuang GAO Mei-xu GUO Wei Hafiz ABDUL HASEEB;ZHANG Jun;GUO Yu-shuang;GAO Mei-xu;GUO Wei

作者机构：Institute of Food Science and TechnologyChinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural AffairsBeijing 100193P.R.China Key Laboratory of Molecular GeneticsChina National Tobacco CorporationGuizhou Institute of Tobacco ScienceGuiyang 550081P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第2期

页      面：446-459页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：partially supported by the National Key Research and Development Program of China(2017YFC1600903 and 2016YFD040015) the National Natural Science Foundation of China(32072377) the Beijing Natural Science Foundation,China(6192023) the Agricultural Science and Technology Innovation Program of China(CAAS-ASTIP-2020-IFST-03) the scholarship grant from the China Scholarship Council(CSC)(2017GXZ022555) 

主　　题：apoplastic proteins maize defense response proteome Fusarium verticillioides 

摘      要：During the attack of a pathogen, a variety of defense-associated proteins are released by the host plant in the apoplast to impede the perceived attack. This study utilized the mass spectrometry(LC-MS/MS) and label-free quantification method to analyze the apoplastic fluid(APF) from maize stalk and identified the proteins responsive to the Fusarium verticillioides infection. We have identified 742 proteins, and among these, 119 proteins were differentially accumulated(DAPs), i.e., 35 up-regulated, 18 down-regulated, and 66 proteins were only induced by the pathogen infection. The differentially accumulated proteins were analyzed for their Gene Ontology(GO) and Kyoto Encyclopedia of Gene and Genomes(KEGG) pathway enrichment. The highly enriched Biological Process(BP) term was the L-serine biosynthesis process, whereas the most enriched Molecular Function(MF) term was the cysteine-type endopeptidase inhibitor activity. It was also found that the pathways related to the biosynthesis of amino acid, biosynthesis of secondary metabolites, protein processing in the endoplasmic reticulum, and carbohydrate metabolic pathways were significantly enriched. Moreover, 61 out of 119 differentially accumulated proteins were predicted as secretory proteins. The secretory pathways analysis showed that a greater number of proteins were secreted through the conventional secretion system compared to the unconventional secretion system. The identified secreted proteins were related to a variety of pathways in defense responses including cell redox homeostasis, cell wall modification, signal transduction, carbohydrate metabolism, binding proteins(metal ion binding, RNA binding and heme-binding), maintenance and stabilization of other proteins, indicating a complex response from the plant to the fungal infection. Our data suggested that a number of host proteins belonging to various pathways have been modulated in the apoplastic region.