Characterization of a new 4,6-α-glucanotransferase from Limosilactobacillus fermentum NCC 3057 with ability of synthesizing low molecular mass isomalto-/maltopolysaccharide

作     者：Weikang Yang Lufei Sheng Sheng Chen Lei Wang Lingqia Su Jing Wu 

作者机构：State Key Laboratory of Food Science and TechnologyJiangnan University1800 Lihu AvenueWuxi214122China School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of EducationJiangnan University1800 Lihu AvenueWuxi214122China International Joint Laboratory on Food SafetyJiangnan University1800 Lihu AvenueWuxi214122China 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2022年第46卷第2期

页      面：241-250页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：This work received financial support from the National Science Fund for the National Natural Science Foundation of China(31730067) the National Natural Science Foundation of China(31972032) the National Natural Science Foundation of China(32001637) 

主　　题：4,6-α-Glucanotransferase Isomalto-/maltopolysaccharide Soluble dietary fiber X-ray crystal structure Molecular mass of product NMR spectroscopy 

摘      要：4,6-α-Glucanotransferase(4,6-α-GT)converts starch into product with increasedα(1-6)glycosidic bonds ratio,and this product is a new type of soluble dietary fiber with property of escaping small intestine digestion.4,6-α-GT gained interest recently because of their potential use in enzymatic synthesis of soluble dietary *** this study,a putative GtfB sequence from Limosilactobacillus fermentum NCC 3057 was *** sequence was truncated and expressed in Escherichia coli to obtain the protein *** NCC 3057 GtfBΔ***ΔN showed optimal activity at 35℃and pH 6.0,and it converted amylose V to isomalto-/maltopolysaccharide(IMMP)with low molecular mass(3.1 kDa).This IMMP product contains 72%α(1-6)glycosidic bonds,and it showed 64%indigestible content in vitro digestion *** results indicate that the product of *** NCC 3057 GtfBΔN is a soluble dietary *** the X-ray crystal structure of GtfBΔN(2.4Å)was *** on the GtfBΔN structure,we offer an insight about that three loops of domain C may be related to the molecular mass of IMMP product.