Study on the Protective Mechanism of Acupuncture Regulating ICAM-5 mRNA Expression in Rats with Ischemic Stroke
Study on the Protective Mechanism of Acupuncture Regulating ICAM-5 mRNA Expression in Rats with Ischemic Stroke作者机构：Xi’an Hospital of Traditional Chinese Medicine Xi’an China Shaanxi University of traditional Chinese Medicine Xianyang China
出 版 物：《Journal of Behavioral and Brain Science》 (行为与脑科学期刊（英文）)
年 卷 期：2021年第11卷第12期
页 面：313-321页
学科分类：1006[医学-中西医结合] 1005[医学-中医学] 100502[医学-中医临床基础] 10[医学] 100602[医学-中西医结合临床]
主 题：Ischemic Stroke Acupuncture ICAM-5 mRNA Mechanism Study
摘 要：Objective: The objective is to investigate whether acupuncture can protect brain by regulating the expression of ICAM-5 mRNA in brain tissue. Methods: Male SD rats were used to construct a middle cerebral artery occlusion reperfusion injury (Middle cerebral artery occlusion, MCAO) model, and the rats were randomly divided into 4 groups, namely the normal group (group A) and the sham operation group (group B), MCAO group (group C), MCAO + acupuncture group (group D);real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of ICAM-5 mRNA in the brain tissue of the ischemic area, and HE staining and TUNEL were used to detect the level of neuronal apoptosis to evaluate the therapeutic effect. Results: Acupuncture can improve neuronal morphology and reduce neuronal apoptosis in rats with ischemic stroke. RT-qPCR: compared with group A and group B, the expression level of ICAM-5 mRNA in the brain tissues of group C and group D decreased, while compared with group C, The expression of ICAM-5 mRNA in the brain tissue of group D was significantly up-regulated (P 0.05). Conclusion: The effectiveness of acupuncture in the treatment of ischemic stroke may achieve neuroprotection by up-regulating the expression level of ICAM-5 mRNA.
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