<i>In Vitro</i>Clonal Propagation from Juvenile and Different Explant Types of Two Edible <i>Annonaceae</i>Species: <i>Annona muricata</i>L. and <i>Annona squamosa</i>L.

作     者：Oumar Ba Abraham Dieme Amadou Lamine Ndoye Mame Ourèye Sy Oumar Ba;Abraham Dieme;Amadou Lamine Ndoye;Mame Ourèye Sy

作者机构：Laboratoire Campus de Biotechnologies Vegetales Departement de Biologie Vegetale Faculte des Sciences & Techniques Universite Cheikh Anta Diop Dakar Senegal Laboratoire Mixte International-Adaptation des Plantes et Microorganismes associes aux Stress Environnementaux (LMI-LAPSE) IRD ISRA UCAD Dakar Senegal 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2021年第12卷第12期

页      面：458-480页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：Annona muricata Annona squamosa Juvenile Material Micropropagation 6-Benzylaminopurine 6-Furfuryl Aminopurine 1-Naphthaleneacetic Acid Indole-3-Butyric Acid 

摘      要：Annona muricata L. and Annona squamosa L. are tropical species whose fleshy fruit is edible. They offer real possibilities for socio-economic use, particularly in the fields of medicine, nutrition, ecosystem conservation and the poverty alleviation. This study was set up to evaluate different methods of micropropagation from juvenile material for the regeneration of these species. Thus, MS medium supplemented with [BAP 2 mg·L-1] i.e. M2 produced 2.87 newly formed shoots from the cotyledonary nodes of A. muricata. For the terminal apices of A. squamosa, it was MMS medium supplemented with [BAP 2 mg·L-1] i.e. MM2 that was most conducive to new shoot formation (3.12). The addition of 0.1 and 0.2 mg·L-1 of NAA in the M2 medium, made it possible to have the best elongations and average number of nodes for the new shoots from cotyledonary nodes of A. muricata (9.11 cm for 5.62 nodes). With A. squamosa, MM7 medium [MMS + BAP 1 mg·L-1+ KIN 1 mg·L-1] resulted in an average length of 9.05 cm with 5.62 nodes on average for the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50 mg·L-1] and 2 g·L-1 of activated charcoal gave a rooting rate of 66.67% for shoots originating from the cotyledonary nodes of A. squamosa;while with vitroplants from cotyledonary nodes of A. muricata, a better rooting rate (83.33%) was obtained following a 5-day rhizogenic induction. After 30 days of acclimatization, the survival rate reached 83.33% for plants from the tips of A. muricata, whe