Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

作     者：Fengjiao Zhu Yahui Ji Jiu Deng Linmei Li Xue Bai Xianming Liu Bingcheng Lin Yao Lu Fengjiao Zhu;Yahui Ji;Jiu Deng;Linmei Li;Xue Bai;Xianming Liu;Bingcheng Lin;Yao Lu

作者机构：Department of BiotechnologyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第6期

页      面：2893-2900页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：the National Natural Science Foundation of China(Nos.21874133,31927802) Youth Innovation Promotion Association CAS(No.2018217) funds from the Dalian Institute of Chemical Physics,CAS(No.I201908) 

主　　题：Microfluidics Single-cell EV analysis Single EV analysis Extracellular vesicle Heterogeneity 

摘      要：Extracellular vesicles(EVs)are membrane vesicles secreted by cells,playing critical roles in mediating intercellular communications for various physiological and pathological *** of the EV analysis is currently performed at the bulk level,obscuring the origin of the EVs and diverse characteristics of the individual extracellular *** to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV *** platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the *** microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis,which would be summarized in this mini-review.