The inhibition mechanisms of pancreatic lipase by apigenin and its anti-obesity mechanisms revealed by using network pharmacology

作     者：Fuqiang Liang Yumeng Shi Weiwei Cao Jiayi Shi 

作者机构：College of Food Science and EngineeringCollaborative Innovation Center for Modern Grain Circulation and SafetyNanjing University of Finance and EconomicsNanjing210023China College of Food Science and BioengineeringHenan University of Science and TechnologyLuoyang471023China 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2022年第45卷第1期

页      面：293-300页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：supported by[National Natural Science Foundation of China](Grant number 32001702) 

主　　题：Anti-Obesity Apigenin Pancreatic lipase Inhibition mechanism Molecular modeling Network pharmacology 

摘      要：This study aimed to elucidate the inhibition mechanism of apigenin against porcine pancreatic lipase(PPL),and,moreover,to comprehensively reveal the molecular basis of its anti-obesity via network pharmacology *** results showed that apigenin inhibited PPL with an IC50 value of 0.377±0.041 *** techniques combined molecular docking suggested that apigenin could bind into the PPL active pocket,affecting its normal spatial ***,molecular dynamic(MD)simulations revealed that the open conformation of PPL tended to transit to the closed in the presence of apigenin,which might one important reason for the inhibition of PPL catalytic *** pharmacology analysis revealed that a total of 49 proteins could be identified as potential targets for the anti-obesity effects of *** to the protein-protein interaction(PPI)network analysis,six hub targets were extracted including IGF1,ESR1,MMP9,PPARA,MAPK14 and *** Encyclopedia of Genes and Genomes(KEGG)pathway enrichment indicated that the 49 potential targets could be mapped to 30 pathways(p0.05).Among them,PI3K-Akt signaling pathway and insulin resistance can be considered as two major pathways regulated by *** docking studies indicated that apigenin can bind into the binding pocket of the six hub target proteins identified according to the PPI *** results indicated that in addition to inhibiting PPL,apigenin could exhibit anti-obesity benefit through the molecular mechanisms uncovered by network *** study proposes a new strategy to reveal the mechanisms of dietary polyphenols at the level of network pharmacology.