iPSC-derived cranial neural crest-like cells can replicate dental pulp tissue with the aid of angiogenic hydrogel

作     者：Yoshifumi Kobayashi Julie Nouet Erdenechimeg Baljinnyam Zain Siddiqui Daniel HFine Diego Fraidenraich Vivek A.Kumar Emi Shimizu 

作者机构：Department of Oral BiologyRutgers School of Dental MedicineNewarkNJ07103USA Department of Cell Biology and Molecular MedicineRutgers New Jersey Medical SchoolNewarkNJ07103USA Department of Biomedical EngineeringNew Jersey Institute of TechnologyNewarkNJ07102USA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第8期

页      面：290-301页

核心收录：

学科分类：1003[医学-口腔医学] 10[医学] 

基　　金：supported by NIH grants,R01DE025885(E.S),R15EY029504(VAK) National Science Foundation NSF IIP 1903617(VAK) 

主　　题：Regenerative endodontics iPSC Cranial neural crest Fibroblast growth factor Odontoblastic differentiation Angiogenic self-assembling peptide hydrogel 

摘      要：The dental pulp has irreplaceable roles in maintaining healthy teeth and its regeneration is a primary aim of regenerative *** study aimed to replicate the characteristics of dental pulp tissue by using cranial neural crest(CNC)-like cells(CNCLCs);these cells were generated by modifying several steps of a previously established method for deriving NC-like cells from induced pluripotent stem cells(iPSCs).CNC is the anterior region of the neural crest in vertebrate embryos,which contains the primordium of dental pulp cells or *** produced CNCLCs showed approximately 2.5-12,000-fold upregulations of major CNC marker ***,the CNCLCs exhibited remarkable odontoblastic differentiation ability,especially when treated with a combination of the fibroblast growth factors(FGFs)FGF4 and *** FGFs induced odontoblast marker genes by 1.7-5.0-fold,as compared to bone morphogenetic protein 4(BMP4)*** a mouse subcutaneous implant model,the CNCLCs briefly fated with FGF4+FGF9 replicated dental pulp tissue characteristics,such as harboring odontoblast-like cells,a dentin-like layer,and vast neovascularization,induced by the angiogenic self-assembling peptide hydrogel(SAPH),*** acts as a versatile biocompatible scaffold in the canal *** study demonstrated a successful collaboration between regenerative medicine and SAPH technology.